Cyclin D1 and p22ack1 play opposite roles in plant growth and development
| Autor: | Sun Chung Park, Jeong Woo Cho, Chong Ki Kim, Woong Han, Myeong Hyeon Wang, Pill Soon Song, Eun Ah Shin, Soo-In Sohn |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cell division
Cyclin D Cyclin A Meristem Biophysics Arabidopsis Cell Cycle Proteins Biology Biochemistry Cyclin D1 Cyclin-dependent kinase Two-Hybrid System Techniques Molecular Biology In Situ Hybridization Arabidopsis Proteins Cell Cycle Cell Biology biology.organism_classification Plants Genetically Modified Cell biology Plant Leaves Phenotype biology.protein Carrier Proteins Cyclin A2 |
| Zdroj: | Biochemical and biophysical research communications. 324(1) |
| ISSN: | 0006-291X |
| Popis: | The plant cell division cycle, a highly coordinated process, is continually regulated during the growth and development of plants. In this report, we demonstrate how two cell-cycle regulators act together to control cellproliferation in transgenic Arabidopsis. To identify potential cyclin dependent kinase regulators from Arabidopsis, we employed an two-hybrid screening system to isolate genes encoding GI specific cyclin-interacting proteins. One of these, p22 a c k 1 , which encodes a novel 22 kDa protein, binds to cyclin D 1, Overexpression of p22 a c k 1 in transgenic Arabi9dopsis resulted in growth retardation due to a strong inhibition of cell division in the leaf primordial and merislematic tissue. The leaf shape of p22 a c k 1 transgenic Arabidopsis was altered from oval in wild-type to dentate. Wild-type phenotype was successfully restored in FI hybrids by cross-hybridizing the p22 a c k 1 Arabidopsis mutants with cyclin D1. Taken together. these results suggest that p22 a c k 1 and cyclin D1, which act antagonistically, are major rate-limiting factors for cell division in the leaf meristem. |
| Databáze: | OpenAIRE |
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