New Insights into the Antioxidant and Anti-Inflammatory Effects of Italian Salvia officinalis Leaf and Flower Extracts in Lipopolysaccharide and Tumor-Mediated Inflammation Models
| Autor: | Rosa Tundis, Monica Rosa Loizzo, Anna Rita Cappello, Chouaha Bouzidi, Brigitte Deguin, Luca Frattaruolo, Annabelle Dugay, Maria Stella Cappello, Matteo Brindisi, Graziantonio Lauria |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Antioxidant Physiology medicine.drug_class DPPH anti-infiammatory activity medicine.medical_treatment Clinical Biochemistry HPLC-DAD-ESI-Q-MS Pharmacology Biochemistry Article Anti-inflammatory antioxidant effects 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food medicine Viability assay Nitrite anti-inflammatory activity Molecular Biology ABTS Chemistry lcsh:RM1-950 Salvia officinalis Cell Biology cancer cell-conditioned media food.food cancer-cell-conditioned media lcsh:Therapeutics. Pharmacology sage 030104 developmental biology 030220 oncology & carcinogenesis Officinalis |
| Zdroj: | Antioxidants Volume 10 Issue 2 Antioxidants, Vol 10, Iss 311, p 311 (2021) Antioxidants 10 (2021): 1–20. doi:10.3390/antiox10020311 info:cnr-pdr/source/autori:Brindisi M.; Bouzidi C.; Frattaruolo L.; Loizzo M.R.; Cappello M.S.; Dugay A.; Deguin B.; Lauria G.; Cappello A.R.; Tundis R./titolo:New insights into the antioxidant and anti-inflammatory effects of italian salvia officinalis leaf and flower extracts in lipopolysaccharide and tumor-mediated inflammation models/doi:10.3390%2Fantiox10020311/rivista:Antioxidants/anno:2021/pagina_da:1/pagina_a:20/intervallo_pagine:1–20/volume:10 |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10020311 |
| Popis: | This work aimed to investigate and compare the in vitro antioxidant and anti-inflammatory effects of Salvia officinalis L. (sage) from Italy, with the aim of raising its current knowledge in this field. Leaves and flowers (S1–S8), harvested in two areas of Southern Italy, were extracted with methanol as a solvent by maceration or ultrasound-assisted extraction. Sage extracts, analysed by high pressure liquid chromatography-diode-array detection-electrospray ionization-quadrupole-mass spectroscopy (HPLC-DAD-ESI-Q-MS), exerted a promising antioxidant activity investigated using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and β-carotene bleaching tests, and elicited a significant decrease in reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. The anti-inflammatory activity was analysed in the same in vitro model. All the extracts did not affect cell viability although they showed anti-inflammatory activity, as they induced a decrease in nitrite levels that was greater than 50%, when employed at 50 µg/mL. Furthermore, they elicited a decrease in nitrite levels, as well as a decline in pro-inflammatory cytokine expression. The NF-κB transcription factor proved to be involved in the mechanisms that underlie such effects. Interestingly, sage extracts were able to interfere with the inflammatory activity induced by breast cancer cell-conditioned media (nitrite levels were significantly decreased, p < 0.05 p < 0.01), highlighting for the first time the important role of S. officinalis in controlling inflammation processes related to neoplastic progression. |
| Databáze: | OpenAIRE |
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