Insulin provokes co-ordinated increases in the synthesis of phosphatidylinositol, phosphatidylinositol phosphates and the phosphatidylinositol–glycan in BC3H-1 myocytes
| Autor: | Robert V. Farese, R J Pollet, Denise R. Cooper, Govindan P. Nair, Mary L. Standaert, T S Konda |
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| Rok vydání: | 1988 |
| Předmět: |
Glycosylphosphatidylinositols
medicine.medical_treatment Phosphatidylinositol Phosphates Phospholipid Stimulation Biology Phosphatidylinositols Biochemistry Cell Line chemistry.chemical_compound Biosynthesis Polysaccharides Glucosamine medicine Insulin Inositol Phosphatidylinositol Molecular Biology Muscles Cell Biology Stimulation Chemical chemistry Chromatography Thin Layer Research Article |
| Zdroj: | Biochemical Journal. 256:185-188 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2560185 |
| Popis: | BC3H-1 myocytes were cultured in the presence of [3H]inositol or [3H]glucosamine during their entire growth cycle to ensure that all lipids containing inositol and glucosamine were labelled to isotopic equilibrium or maximal specific radioactivity. After such labelling, a lipid (or group of lipids), which was labelled with both inositol and glucosamine, was observed to migrate between phosphatidylinositol 4-phosphate and phosphatidylinositol (PI) in two different t.l.c. systems. Insulin provoked rapid, sizeable, increases in the inositol-labelling of this lipid (presumably a PI-glycan), and these increases were similar to those observed in PI and PI phosphates. Our results indicate that insulin provokes co-ordinated increases in the net synthesis de novo of PI and its derivatives, PI phosphates and the PI-glycan, in BC3H-1 myocytes. This increase in synthesis of PI may serve as the mechanism for replenishing the PI-glycan during stimulation of its hydrolysis by insulin. Moreover, increases in the content of the PI-glycan may contribute to increases in the generation of head-group ‘mediators’ during insulin action. |
| Databáze: | OpenAIRE |
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