| Autor: |
Craig A. Watkins, Taylor Gaines, Fiona Strathdee, Johanna L. Baily, Eleanor Watson, Ailsa J. Hall, Andrew Free, Mark P. Dagleish |
| Přispěvatelé: |
University of St Andrews. School of Biology, University of St Andrews. Sea Mammal Research Unit, University of St Andrews. Scottish Oceans Institute, University of St Andrews. Marine Alliance for Science & Technology Scotland |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Watkins, C, Gaines, T, Strathdee, F, Baily, J, Watson, E, Hall, A J, Free, A & Dagleish, M P 2022, ' A comparative study of the fecal microbiota of gray seal pups and yearlings-a marine mammal sentinel species ', MicrobiologyOpen, vol. 11, no. 3 . https://doi.org/10.1002/mbo3.1281 |
| ISSN: |
2045-8827 |
| DOI: |
10.1002/mbo3.1281 |
| Popis: |
Illumina DNA sequencing was carried out by Edinburgh Genomics at the University of Edinburgh, which is partly supported through core grants from NERC (R8/H10/56), MRC (MR/K001744/1), and BBSRC (BB/J004243/1). C. A. W., E. W., and M. P. D. received funding from the Scottish Government Rural & Environment Science & Analytical Services (RESAS). This study and J. L. B.'s PhD studentship were funded by the Moredun Research Institute and the Royal Zoological Society of Scotland. Gray seals (Halichoerus grypus) can act as sentinel species reflecting the condition of the environment they inhabit. Our previous research identified strains of pathogenic Campylobacter and Salmonella, originating from both human and agricultural animal hosts, on rectal swabs from live gray seal (H. grypus) pups and yearlings on the Isle of May, Scotland, UK. We examined rectal swabs from the same pup (n = 90) and yearling (n = 19) gray seals to gain further understanding into the effects of age-related changes (pup vs. yearling) and three different natal terrestrial habitats on seal pup fecal microbiota. DNA was extracted from a subset of rectal swabs (pups n = 23, yearlings n = 9) using an optimized procedure, and the V4 region of the 16S ribosomal RNA gene was sequenced to identify each individual's microbiota. Diversity in pup samples was lower (3.92 ± 0.19) than yearlings (4.66 ± 0.39) although not significant at the p = 0.05 level (p = 0.062) but differences in the composition of the microbiota were (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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