Listeria monocytogenes Scott A: cell surface charge, hydrophobicity, and electron donor and acceptor characteristics under different environmental growth conditions
| Autor: | Thierry Meylheuc, Romain Briandet, Catherine Maher, Marie Noelle Bellon-Fontaine |
|---|---|
| Přispěvatelé: | Bioadhésion et Hygiène des Matériaux (BHM), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, SKW Biosystems, University College Cork (UCC) |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
croissance bactérienne
surface cellulaire Sodium intéraction électrostatique chemistry.chemical_element Electrons Electron donor Applied Microbiology and Biotechnology Bacterial Adhesion mobilité électrophorétique Electron Transport 03 medical and health sciences chemistry.chemical_compound Microelectrophoresis température Humans Yeast extract Surface charge 030304 developmental biology [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences adhésion microbienne Ecology 030306 microbiology Cell Membrane Temperature Stainless Steel Listeria monocytogenes Acceptor Culture Media hydrophobicité Lactic acid Biochemistry chemistry propriété physicochimique Solvents Food Microbiology Brain heart infusion listeria monocytogènes Food Science Biotechnology Nuclear chemistry |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 1999, 65 (12), pp.5328-5333 Scopus-Elsevier HAL Applied and Environmental Microbiology 12 (65), 5328-5333. (1999) |
| ISSN: | 0099-2240 1098-5336 |
| Popis: | We determined the variations in the surface physicochemical properties of Listeria monocytogenes Scott A cells that occurred under various environmental conditions. The surface charges, the hydrophobicities, and the electron donor and acceptor characteristics of L. monocytogenes Scott A cells were compared after the organism was grown in different growth media and at different temperatures; to do this, we used microelectrophoresis and the microbial adhesion to solvents method. Supplementing the growth media with glucose or lactic acid affected the electrical, hydrophobic, and electron donor and acceptor properties of the cells, whereas the growth temperature (37, 20, 15, or 8°C) primarily affected the electrical and electron donor and acceptor properties. The nonlinear effects of the growth temperature on the physicochemical properties of the cells were similar for cells cultivated in two different growth media, but bacteria cultivated in Trypticase soy broth supplemented with 6 g of yeast extract per liter (TSYE) were slightly more hydrophobic than cells cultivated in brain heart infusion medium ( P < 0.05). Adhesion experiments conducted with L. monocytogenes Scott A cells cultivated in TSYE at 37, 20, 15, and 8°C and then suspended in a sodium chloride solution (1.5 × 10 −1 or 1.5 × 10 −3 M NaCl) confirmed that the cell surface charge and the electron donor and acceptor properties of the cells had an influence on their attachment to stainless steel. |
| Databáze: | OpenAIRE |
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