Response of dairy calves to vaccinia viruses that express foreign genes
| Autor: | C M Geissinger, E Paoletti, W P Higgins, M Perkus, F W Scott, S Mercer, J H Gillespie, D F Holmes |
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| Rok vydání: | 1986 |
| Předmět: |
Male
Microbiology (medical) Scab formation viruses Genetic Vectors DNA Recombinant Cattle Diseases Vaccinia virus Antibodies Viral medicine.disease_cause Virus Microbiology chemistry.chemical_compound Viral Envelope Proteins Neutralization Tests Vaccinia medicine Animals Poxviridae Orthopoxvirus Hepatitis B Antibodies Neutralizing antibody Hepatitis B virus Hepatitis B Surface Antigens biology biology.organism_classification Virology Herpes simplex virus chemistry biology.protein Cattle Female Immunization Immunologic Memory Research Article |
| Zdroj: | Journal of Clinical Microbiology. 23:283-288 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.23.2.283-288.1986 |
| Popis: | Repeated intradermal inoculations of calves with wild-type vaccinia virus and recombinant vaccinia viruses expressing human hepatitis B virus surface antigen and herpes simplex virus, type 1, glycoprotein D produced characteristic pox lesions at each site of injection. In some instances, calves were inoculated as many as five times at intervals from 4 to 7 weeks. The lesions invariably were more severe after the second inoculation. Subsequent inoculations produced a less severe area of redness, swelling, necrosis, and scab formation. No other signs of illness, such as an elevation in temperature, were noted in the calves. Vaccinia virus was isolated in low titers from scabs taken at various times after inoculation. No lesions were formed at the sites injected with tissue culture fluid and cellular debris at the same time that virus inoculations were made. Calf contact controls remained normal through the 8-week exposure in isolation units with calves inoculated twice with vaccinia virus. No neutralizing antibody to vaccinia virus was detected in the contact controls. In contrast, the virus-inoculated calves developed neutralizing antibody to vaccinia virus and to herpes simplex virus glycoprotein D in serum. In all cattle, a second inoculation significantly enhanced the neutralizing antibody response within 1 week, suggesting that an anamnestic response had occurred. No antibody to hepatitis B virus surface antigen was elicited in calves after repeated inoculations with vaccinia recombinants that express hepatitis B virus surface antigen and are known to elicit in rabbits antibodies reactive with hepatitis B virus surface antigen. |
| Databáze: | OpenAIRE |
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