Ultradeep Sequencing for Detection of Quasispecies Variants in the Major Hydrophilic Region of Hepatitis B Virus in Indonesian Patients
| Autor: | Yoshitake Hayashi, Yoshiki Murakami, Doddy Widjanarko, Maria Inge Lusida, Juniastuti, Laura Navika Yamani, Takeshi Azuma, Yoshihiko Yano, Yujiao Liang, Takako Utsumi, Soetjipto, Toshihito Tanahashi, Widya Wasityastuti, Ari Triantanoe, Rina Okada, Hadi Wandono |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Microbiology (medical) Hepatitis B virus HBsAg Population Viral quasispecies medicine.disease_cause Liver disease Gene Frequency Virology medicine Humans education Aged education.field_of_study Hepatitis B Surface Antigens biology Genetic Variation High-Throughput Nucleotide Sequencing Sequence Analysis DNA Middle Aged Hepatitis B medicine.disease Titer Amino Acid Substitution Indonesia Immunology biology.protein Female Antibody |
| Zdroj: | Journal of Clinical Microbiology. 53:3165-3175 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Quasispecies of hepatitis B virus (HBV) with variations in the major hydrophilic region (MHR) of the HBV surface antigen (HBsAg) can evolve during infection, allowing HBV to evade neutralizing antibodies. These escape variants may contribute to chronic infections. In this study, we looked for MHR variants in HBV quasispecies using ultradeep sequencing and evaluated the relationship between these variants and clinical manifestations in infected patients. We enrolled 30 Indonesian patients with hepatitis B infection (11 with chronic hepatitis and 19 with advanced liver disease). The most common subgenotype/subtype of HBV was B3/adw (97%). The HBsAg titer was lower in patients with advanced liver disease than that in patients with chronic hepatitis. The MHR variants were grouped based on the percentage of the viral population affected: major, ≥20% of the total population; intermediate, 5% to |
| Databáze: | OpenAIRE |
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