Bone morphogenetic protein-4 abrogates lumen formation by mammary epithelial cells and promotes invasive growth

Autor: Roberto Montesano
Rok vydání: 2007
Předmět:
Neoplasm Invasiveness/physiopathology
Cell Transformation
Neoplastic/pathology
medicine.medical_treatment
Mammary gland
Biophysics
Morphogenesis
Lumen (anatomy)
Bone Morphogenetic Protein 4
Biology
Bone morphogenetic protein
Biochemistry
Mice
Mammary Glands
Animal
Nitriles
Butadienes
medicine
Animals
Humans
Neoplasm Invasiveness
ddc:612
Cell adhesion
Molecular Biology
Cells
Cultured
Imidazoles/pharmacology
Anthracenes
Bone Morphogenetic Proteins/pharmacology
Butadienes/pharmacology
Imidazoles
Epithelial Cells
Mitogen-Activated Protein Kinases/antagonists & inhibitors
Cell Biology
Epithelium
Cell biology
Anthracenes/pharmacology
Cell Transformation
Neoplastic
medicine.anatomical_structure
Cytokine
Bone morphogenetic protein 4
Mammary Glands
Animal/cytology
Nitriles/pharmacology
Bone Morphogenetic Proteins
Immunology
Epithelial Cells/drug effects/pathology
Female
Mitogen-Activated Protein Kinases
Zdroj: Biochemical and Biophysical Research Communications, Vol. 353, No 3 (2007) pp. 817-22
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2006.12.109
Popis: Bone morphogenetic proteins (BMPs) are multifunctional cytokines that regulate key developmental processes, but are also overexpressed in many carcinomas. To assess whether BMPs would influence the three-dimensional architecture of epithelial structures, we took advantage of an in vitro model in which mammary epithelial cells form alveolar-like spherical cysts in collagen gels. We found that BMP-4 has a dramatic, biphasic effect on the organization of epithelial cysts. When added in the concentration range of 1-10 ng/ml, the cytokine abrogates lumen formation and induces the outgrowth of multiple invasive cord-like structures. At higher concentrations (20-100 ng/ml), BMP-4 additionally disrupts cell-cell adhesion, resulting in cyst disintegration and scattering of individual cells into the surrounding collagen matrix. The finding that BMP-4 subverts the ability of mammary epithelial cells to form polarized lumen-containing structures and endows them with invasive properties supports the involvement of this cytokine in the progression of breast cancer.
Databáze: OpenAIRE
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