MRSA genotypes in Turkey: Persistence over 10 years of a single clone of ST239
| Autor: | Corné H. W. Klaassen, Cüneyt Özakin, Mehmet Doganay, Çiğdem Kuzucu, Kemalettin Aydin, Ulku Altoparlak, Özge Turhan, Aynur Engin, Mehmet Ali Özinel, Emine Alp, Andreas Voss |
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| Přispěvatelé: | Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Klinik Mikrobiyoloji., Özakın, Cüneyt, AAG-8392-2021, [Alp, Emine -- Doganay, Mehmet] Erciyes Univ, Dept Infect Dis & Clin Microbiol, TR-38039 Kayseri, Turkey -- [Klaassen, Corne H. W.] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands -- [Altoparlak, Ulku] Ataturk Univ, Erzurum, Turkey -- [Aydin, Kemalettin] Karadeniz Tech Univ, Dept Infect Dis & Clin Microbiol, Trabzon, Turkey -- [Engin, Aynur] Cumhuriyet Univ, Dept Infect Dis & Clin Microbiol, Sivas, Turkey -- [Kuzucu, Cigdem] Inonu Univ, Dept Microbiol & Clin Microbiol, Malatya, Turkey -- [Ozakin, Cuneyt] Uludag Univ, Bursa, Turkey -- [Ozinel, Mehmet Ali] Ege Univ, Izmir, Turkey -- [Turhan, Ozge] Akdeniz Univ, Dept Infect Dis & Clin Microbiol, TR-07058 Antalya, Turkey -- [Voss, Andreas] Canisius Wilhelmina Hosp, Nijmegen, Netherlands -- [Voss, Andreas] Radboud Univ Nijmegen, Med Ctr, NL-6525 ED Nijmegen, Netherlands |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Bacterium identification Meticillin Electrophoresis gel pulsed-field Bacterial toxins Turkey Epidemiology Antibiotic resistance Bacterial meningitis Prevalence Strains Bacterial strain medicine.disease_cause Turkey (republic) Staphylococcus infection Bacterial proteins Leukocidins Bacterial genetics Genotype Region Staphylococcal infections Child Middle aged Genetic similarity Panton-Valentine Leukocidin Methicillin Resistant Staphylococcus Aureus Staphylococcal Infections Oxacillin Aged 80 and over Community-acquired infections Methicillin-resistant Bacterial arthritis Pathogenesis and modulation of inflammation [N4i 1] Pcr Pulsed field gel electrophoresis Staphylococcus aureus Molecular epidemiology Infectious diseases Methicillin-resistant Staphylococcus aureus Female medicine.drug Resistant staphylococcus-aureus Field gel-electrophoresis Human Microbiology (medical) Adult Bacterium isolate Adolescent Child preschool Disease susceptibility Clone Multilocus sequence typing Exotoxins Major clinical study Biology Infections Article Microbiology Cross infection Sepsis medicine Pulsed-field gel electrophoresis Penicillin-Binding Proteins Humans Typing Panton Valentine leucocidin genes Prospective study Aged Infant Pneumonia biochemical phenomena metabolism and nutrition Valentine leukocidin genes bacterial infections and mycoses Nonhuman Young adult Lung abscess Antibiotic sensitivity Genetic association Methicillin-resistant staphylococcus aureus Geographic distribution Prospective studies Controlled study Panton Valentine leukocidin Methicillin resistant Staphylococcus aureus |
| Zdroj: | Journal of Infection, 58, 433-8 Journal of Infection, 58, 6, pp. 433-8 |
| ISSN: | 0163-4453 |
| Popis: | WOS: 000267297400005 PubMed ID: 19446883 Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of life-threatening human infections. The clinical impact of MRSA is mounting, not only due to the ever-increasing prevalence but also due to the occurrence of new, community-acquired MRSA strains. The aim of this prospective, multi-centre study was to determine the prevalence and genetic relatedness of clinically relevant MRSA isolates, in Turkey. Methods: During a 1-year period, data from 20 successive patients with invasive S. aureus infections were collected from eight university hospitals, geographically distributed over the six main regions of Turkey. Among these S. aureus isolates, the genetic association of MRSA isolates was investigated by pulsed-field get electrophoresis (PFGE) and spa typing. A selected number of isolates were also analyzed by multilocus sequence typing (MLST). Furthermore, Panton Valentine leucocidin (PVL) genes were examined. Results: In this study, the rate of methicillin resistance in S. aureus in patients with apparent infections (sepsis, meningitis, lung abscess or septic arthritis) ranged from 12 to 75% within the seven participating centres. Typing by pulsed-field get electrophoresis and spa typing revealed the presence of 22 closely related genotypes. According to the PFGE and spa typing results, 53 out of 54 MRSA isolates were closely related. These isolates were of spa type t030 or a related spa type, contain an SCC mec type III element and belong to sequence type ST239. None of the isolates contained the PVL genes. Conclusions: Despite the broad surface area of Turkey, a single predominant clone of ST239 circulates in hospitals in different regions and only few new types of MRSA were introduced over the past years. These results place Turkey in the epicenter of ST239 prevalence. (C) 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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