Evaluation of toxicological endpoints in female zebrafish after bisphenol A exposure

Autor: Rosario Moyano, Nahum Ayala, Antonio J. Lora, A. Molina, J. G. Monterde, Noelia Morales-Prieto, Nieves Abril
Rok vydání: 2017
Předmět:
0301 basic medicine
endocrine system
food.ingredient
Follicular Atresia
Down-Regulation
010501 environmental sciences
Endocrine Disruptors
Toxicology
Real-Time Polymerase Chain Reaction
01 natural sciences
Andrology
03 medical and health sciences
Vitellogenin
chemistry.chemical_compound
Vitellogenins
food
Aromatase
Downregulation and upregulation
Phenols
Yolk
Follicular phase
Animals
RNA
Messenger
Benzhydryl Compounds
Zebrafish
0105 earth and related environmental sciences
Atretic Follicle
biology
Dose-Response Relationship
Drug
Estradiol
urogenital system
Chemistry
Follicular atresia
Ovary
General Medicine
Zebrafish Proteins
Up-Regulation
030104 developmental biology
Xenoestrogen
biology.protein
Female
hormones
hormone substitutes
and hormone antagonists
Biomarkers
Water Pollutants
Chemical
Food Science
Environmental Monitoring
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 112
ISSN: 1873-6351
Popis: Given the importance of bisphenol A (BPA) as a xenoestrogen and its potential effects on human and animal health, we evaluated BPA exposure's short-term effects on follicular development, yolk protein vitellogenin (VTG) production and aromatase expression in female zebrafish. Histological modifications were observed along with increased presence of atretic follicles. Whole-body VTG concentration increased with the dose of BPA exposure. In contrast, expression of Cyp19a mRNA in the ovaries of BPA-exposed fish exhibited an apparent non-monotonic response curve, marked by downregulation at 1 μg/L BPA, upregulation at 10 μg/L BPA, and a return to downregulation at 100 μg/L BPA and higher doses. Ovaries only exhibited significant increases in follicular atresia and VTG concentration after exposure to 100 μg/L BPA and higher doses. Ovarian histopathology, aromatase Cyp19a transcript levels and whole-body VTG protein abundance may be good biomarkers for early detection of environmental BPA exposure.
Databáze: OpenAIRE
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