Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
| Autor: | R. Pinheiro-Silva, Ana Domingos, J. de la Fuente, Joana Couto, V. E. Do Rosário, Sandra Antunes |
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| Přispěvatelé: | Fundação para a Ciência e a Tecnologia (Portugal), Instituto de Higiene e Medicina Tropical (IHMT), Global Health and Tropical Medicine (GHTM), Vector borne diseases and pathogens (VBD) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Plasmodium berghei Science 030231 tropical medicine Ion Pumps Plasmodium Biochemistry Genetics and Molecular Biology (miscellaneous) Article Salivary Glands Transcriptome 03 medical and health sciences Mice 0302 clinical medicine SDG 3 - Good Health and Well-being stomatognathic system RNA interference Anopheles parasitic diseases Animals Homeostasis Pathogen Gene Anopheles stephensi Gene knockdown Multidisciplinary Genes Essential biology Sequence Analysis RNA Gene Expression Profiling fungi biology.organism_classification Virology 3. Good health Insect Vectors Malaria 030104 developmental biology Infectious Diseases Gene Knockdown Techniques Insect Science Medicine Insect Proteins Parasitology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| Popis: | Malaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P. berghei-infected mice. The A. stephensi solute carriers prestinA and NDAE1 were up-regulated in response to infection. These molecules are predicted to interact with each other, and are reportedly involved in the maintenance of cell homeostasis. To further evaluate their functions in mosquito survival and parasite infection, these genes were knocked down by RNA interference. Knockdown of prestinA and NDAE1 resulted in reduction of the number of sporozoites in mosquito SGs. Moreover, NDAE1 knockdown strongly impacted mosquito survival, resulting in the death of half of the treated mosquitoes. Overall, our findings indicate the importance of prestinA and NDAE1 in interactions between mosquito SGs and Plasmodium, and suggest the need for further research. Tis work was partially supported by Fundação para a Ciência e a Tecnologia - Global Health and Tropical Medicine (FCT-GHTM) [UID/Multi/04413/2013]. |
| Databáze: | OpenAIRE |
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