A synthetic biological secondary metabolite, Lycogen™, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy
| Autor: | Zhi-Hong Wen, Cheng-Chin Wang, Wen-Sheng Liu, Jyun-Ying Huang, Hui-Min David Wang, Shu-Hung Huang, Shi-Ying Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Lysine lcsh:TX341-641 Response surface methodology (RSM) Ferementation Rhodobacter sphaeroides WL-APD911 Lycogen™ Secondary metabolite biology.organism_classification 01 natural sciences 03 medical and health sciences Rhodobacter sphaeroides 030104 developmental biology 010608 biotechnology SCALE-UP Cell density Botany medicine Yeast extract Fermentation Food science Response surface methodology lcsh:Nutrition. Foods and food supply Food Science medicine.drug |
| Zdroj: | Food science and human wellness, 6(4):195-201 Food Science and Human Wellness, Vol 6, Iss 4, Pp 195-201 (2017) |
| Popis: | The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen⢠production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen⢠invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen⢠yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the Lycogen⢠optimal culture mediums, scale-up procedures and efficient extractions from R. sphaeroides WL-APD911. Keywords: Rhodobacter sphaeroides WL-APD911, Lycogenâ¢, Response surface methodology (RSM), Ferementation |
| Databáze: | OpenAIRE |
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