Determination of fluvoxamine in human plasma by high-performance liquid chromatography with fluorescence detection
| Autor: | Aqeel A. Fatmi, Robert H. Pullen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Dansyl Compounds
Quality Control Chromatography Chemistry Dansyl chloride Ethyl acetate Reproducibility of Results Fluvoxamine Nortriptyline General Chemistry Fluorescence High-performance liquid chromatography chemistry.chemical_compound Spectrometry Fluorescence Blood plasma medicine Humans Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 574:101-107 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(92)80103-w |
| Popis: | Fluvoxamine and nortriptyline, the assay internal standard, were extracted from plasma with ethyl acetate, then reacted with dansyl chloride. The derivatives were quantitated by isocratic reversed-phase high-performance liquid chromatography with fluorescence detection. The assay calibration range for fluvoxamine was 10-1000 ng/ml using a 1-ml plasma sample. Pooled plasma quality control sample relative recoveries at 25 and 250 ng/ml were 103 and 105%, respectively. Estimates of quality control inter-day precision during validation were less than or equal to 3% relative standard deviation. The assay was cross-validated with a gas chromatographic method and has been employed in therapeutic drug level monitoring. |
| Databáze: | OpenAIRE |
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