Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing
| Autor: | Fengti Qiu, Jinxing Liu, Chenxiao Xue, Tuanyao Chai, Caixia Gao, Sinian Xing, Kunling Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Gene Editing
Silent mutation Regeneration (biology) Computational biology Biology Plants Genetically Modified General Biochemistry Genetics and Molecular Biology MicroRNAs Transformation (genetics) Target site Genome editing Gene Expression Regulation Plant CRISPR-Cas Systems Common wheat General Agricultural and Biological Sciences Genome Plant Triticum General Environmental Science |
| Zdroj: | Science China Life Sciences. 65:731-738 |
| ISSN: | 1869-1889 1674-7305 |
| Popis: | Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. |
| Databáze: | OpenAIRE |
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