Monitoring 'promiscuous' drug effects on single cells of multiple cell types
| Autor: | Yina Kuang, David R. Walt |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Drug
Isopropyl Thiogalactoside Cell type media_common.quotation_subject Mitomycin Cell Green Fluorescent Proteins Biophysics Drug Evaluation Preclinical Genomics Living cell Biology Biochemistry medicine Escherichia coli Combinatorial Chemistry Techniques Fiber Optic Technology Molecular Biology media_common Biological activity Cell Biology Gene Expression Regulation Bacterial Microarray Analysis Cell biology Kinetics Rec A Recombinases medicine.anatomical_structure Lac Operon Homogeneous Cell culture DNA Damage Mutagens |
| Zdroj: | Analytical biochemistry. 345(2) |
| ISSN: | 0003-2697 |
| Popis: | Recent advances in genomics and molecular pathogenesis studies have determined that many diseases are caused by a multiplicity of factors. New drug regimens may consist of multiple biologically active agents designed to act synergistically on multiple biochemical targets. Live cell assays are becoming a standard for identifying new drug candidates with an emphasis on "homogeneous" living cell assays in which multiple cell lines are mixed and monitored simultaneously. In this study, we used a high-density single living cell array, based on an optical imaging fiber bundle microwell array, to simultaneously monitor "promiscuous" drug effects on single cells of multiple cell types. Such a capability allows for a more comprehensive understanding of how cells dynamically respond to combinatorial drug libraries or how different cellular pathways and regulation circuits respond cooperatively to drugs in individual cells. |
| Databáze: | OpenAIRE |
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