Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study
| Autor: | Jin Ho Lim, Hyejin Yoon, Hyungjun Kim, San Hyun Yoon, Won Don Lee, Jung Mi Jang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Biology Cleavage (embryo) Insemination Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine Multinucleate Human fertilization In vitro fertilization medicine reproductive and urinary physiology 030219 obstetrics & reproductive medicine In vitro fertilisation urogenital system Time-lapse imaging Embryogenesis Embryo 030104 developmental biology Reproductive Medicine embryonic structures Original Article |
| Zdroj: | Clinical and Experimental Reproductive Medicine |
| ISSN: | 2233-8241 2233-8233 |
| Popis: | Objective We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. Methods A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination. Results No significant differences were observed between the two groups regarding clinical outcomes (p>0.05). ICSI-derived embryos showed significantly faster morphokinetics than those derived from conventional IVF, from the time to pronuclear fading to the time to 6 cells (p0.05). There were no differences in abnormal cleavage events between the two groups (p>0.05); they showed the same rates of direct cleavage from 1 to 3 cells, 2 multinucleated cells, 2 uneven cells, and reverse cleavage. Conclusion The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. However, these differences did not affect the clinical outcomes of the embryo. Additionally, no significant differences in abnormal cleavage events were found according to the fertilization method. |
| Databáze: | OpenAIRE |
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