Small numbers of residual tumor cells at the site of primary inoculation are critical for anti-tumor immunity following challenge at a secondary location
| Autor: | Takashi Kakinuma, Yasmine Belkaid, Hari Nadiminti, Samuel T Hwang, Takashi Murakami, Bradford A. Perez, Anke S. Lonsdorf, Gulnar Pothiawala, Steven E. Finkelstein, Hisataka Kobayashi |
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| Rok vydání: | 2006 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cancer Research Pathology medicine.medical_specialty Neoplasm Residual Skin Neoplasms Immunology Melanoma Experimental chemical and pharmacologic phenomena Biology Mice Interleukin 21 T-Lymphocyte Subsets Immunity Cell Line Tumor medicine Animals Immunology and Allergy Neoplasm IL-2 receptor neoplasms integumentary system Reverse Transcriptase Polymerase Chain Reaction Melanoma Interleukin-2 Receptor alpha Subunit FOXP3 Forkhead Transcription Factors hemic and immune systems Flow Cytometry medicine.disease Molecular biology Oncology Cell culture Disease Progression Interleukin 12 Female Neoplasm Transplantation |
| Zdroj: | Cancer Immunology, Immunotherapy. 56:1119-1131 |
| ISSN: | 1432-0851 0340-7004 |
| DOI: | 10.1007/s00262-006-0253-4 |
| Popis: | Luciferase-transduced B16 murine melanoma cells (luc-B16) inoculated in ear skin do not form tumors but prevent tumor formation by luc-B16 cells injected into the footpad. To determine the requirements for such immunity, we followed the fate of luc-B16 cells following ear injection. Surprisingly, small numbers of viable luc-B16 cells were detected in tumor-free mouse skin for up to 60 days post-inoculation. After 1 week, the number of Foxp3(+)CD4(+)CD25(+) T cells (along with foxp3 mRNA expression) increased rapidly in the injected ear skin. Residual tumor cells in ears were reduced in mice treated with anti-CD25 mAb and in CD4-deficient mice, but increased in CD8-deficient mice. Strikingly, the loss of luc-B16 cells in the ear skin, either spontaneously or following amputation of the injected ear, resulted in significantly enhanced tumor formation by parental and luciferase-expressing B16 cells after footpad injection. These studies suggest that small numbers of tumor cells (possibly regulated by CD4(+)CD25(+) regulatory T cells expressing Foxp3) are required for effective host anti-tumor responses at alternate inoculation sites. |
| Databáze: | OpenAIRE |
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