Stimulation of Adrenal Chromaffin Cell Proliferation by Hypercalcemia Induced by Intravenous Infusion of Calcium Gluconate in Rats
| Autor: | Etsuko Fujii, Kentaro Asanuma, Atsuhiko Kato, Kenji Adachi, Tetsuro Sugimoto, Shun-ichiro Komatsu, Kaori Isobe, Chie Kato, Tsuneo Ito, Masami Suzuki |
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| Rok vydání: | 2012 |
| Předmět: |
endocrine system
medicine.medical_specialty Short Communication chemistry.chemical_element Stimulation Calcium Toxicology Pathology and Forensic Medicine chemistry.chemical_compound Internal medicine chromaffin cell Medicine rat Carcinogen Calcium metabolism adrenal glands cell proliferation activity business.industry Cell growth hypercalcemia bromodeoxyuridine Endocrinology medicine.anatomical_structure chemistry Chromaffin cell business Adrenal medulla Bromodeoxyuridine |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| DOI: | 10.1293/tox.25.281 |
| Popis: | Increased incidence of adrenal pheochromocytoma is frequently encountered in rat carcinogenicity studies. In some of the studies, the finding is judged to be due to a rat-specific mechanism of carcinogenesis caused by a disturbance of calcium homeostasis. However, direct evidence that the proliferation of chromaffin cells in the adrenal medulla is induced solely by hypercalcemia is not available. In this study, calcium gluconate was intravenously infused for 7 days to rat chromaffin cells by a tail cuff method, and cumulative labeling with bromodeoxyuridine (BrdU) was carried out to evaluate the proliferative activity. The serum calcium concentration was dose-dependently increased, and a high calcium concentration was stably sustained from day 2 to 7. In the adrenal medulla, BrdU-positive chromaffin cells increased in the calcium gluconate-treated animals, and the BrdU-labeling index increased in a dose-dependent manner. In addition, an increased BrdU-labeling index of chromaffin cells was shown to correlate with the serum calcium concentration. Our results demonstrate that hypercalcemia directly enhances the proliferative activity of chromaffin cells and that the proliferative activity is correlated with the serum calcium concentration. |
| Databáze: | OpenAIRE |
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