Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality
| Autor: | Sebastian Böttcher, Andrés C. García-Montero, María Jara-Acevedo, Noemí Muñoz-García, María Belén Vidriales, Jacques J.M. van Dongen, Paloma Bárcena, Margarida Lima, María Tabernero, Antonio López, Anton W. Langerak, Alberto Orfao, Artur Paiva, Quentin Lecrevisse, Julia Almeida, Maria Luz Sanchez |
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| Přispěvatelé: | Immunology, Instituto de Salud Carlos III, European Commission, Junta de Castilla y León, Ministerio de Economía y Competitividad (España), Red Temática de Investigación Cooperativa en Cáncer (España) |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Imunofenotipagem Cell Lymphoproliferative disorders ComputingMilieux_LEGALASPECTSOFCOMPUTING clonality NK cells CD38 CLPD-NK Flow cytometry Immunophenotyping Doenças Linfoproliferativas medicine Células Matadoras Naturais Cluster Analysis Humans Receptor NK-cell clonality Aged Aged 80 and over natural killer cells medicine.diagnostic_test business.industry Chronic lymphoproliferative disorders of NK-cells Middle Aged medicine.disease Flow Cytometry Phenotype Lymphoproliferative Disorders immunophenotype Clone Cells Androgen receptor Killer Cells Natural medicine.anatomical_structure Oncology Receptors Androgen Immunology Chronic Disease Female business Biomarkers Research Paper |
| Zdroj: | Oncotarget Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP Oncotarget, 6(40), 42938-42951. Impact Journals LLC GREDOS. Repositorio Institucional de la Universidad de Salamanca instname Digital.CSIC. Repositorio Institucional del CSIC ResearcherID |
| ISSN: | 1949-2553 |
| Popis: | This is an open-access article distributed under the terms of the Creative Commons Attribution License. Currently, the lack of a universal and specific marker of clonality hampers the diagnosis and classification of chronic expansions of natural killer (NK) cells. Here we investigated the utility of flow cytometric detection of aberrant/altered NK-cell phenotypes as a surrogate marker for clonality, in the diagnostic work-up of chronic lymphoproliferative disorders of NK cells (CLPD-NK). For this purpose, a large panel of markers was evaluated by multiparametric flow cytometry on peripheral blood (PB) CD56 NK cells from 60 patients, including 23 subjects with predefined clonal (n = 9) and polyclonal (n = 14) CD56 NK-cell expansions, and 37 with CLPD-NK of undetermined clonality; also, PB samples from 10 healthy adults were included. Clonality was established using the human androgen receptor (HUMARA) assay. Clonal NK cells were found to show decreased expression of CD7, CD11b and CD38, and higher CD2, CD94 and HLADR levels vs. normal NK cells, together with a restricted repertoire of expression of the CD158a, CD158b and CD161 killer-associated receptors. In turn, NK cells from both clonal and polyclonal CLPD-NK showed similar/overlapping phenotypic profiles, except for high and more homogeneous expression of CD94 and HLADR, which was restricted to clonal CLPD-NK. We conclude that the CD94/HLADR phenotypic profile proved to be a useful surrogate marker for NK-cell clonality. This work has been partially supported by the following grants: FIS 02/1244-FEDER, DTS 15/00119-FEDER, RTICC RD06/0020/0035-FEDER and RTICC RD12/0036/0048-FEDER from the Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain; SA103/03 and SA079U14 from the Consejería de Educación, Junta de Castilla y León, Valladolid, Spain. The research activities of the EuroFlow Consortium were supported by the European Commission (grant STREP EU-FP6, LSHB-CT-2006–018708, entitled ‘Flow cytometry for fast and sensitive diagnosis and follow-up of hematological malignancies’). |
| Databáze: | OpenAIRE |
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