A rapid CE-potential gradient detection method for determination of quinolones
| Autor: | Yongxia Fan, Weidong Qin, Shuo Li, Xiao Gan |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Enoxacin
Ofloxacin Fleroxacin Clinical Biochemistry Quinolones Pharmaceutical formulation Biochemistry Analytical Chemistry chemistry.chemical_compound Oxazines Pazufloxacin Methods medicine Animals Phosphoric acid Detection limit Chromatography Extraction (chemistry) Electrophoresis Capillary Hexane Milk chemistry Fluoroquinolones medicine.drug |
| Zdroj: | ELECTROPHORESIS. 28:4101-4107 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | A rapid CE coupled with potential gradient detection (PGD) for the separation and detection of four quinolones, namely, enoxacin, ofloxacin (OFL), fleroxacin, and pazufloxacin, was described. Separation was performed in a fused-silica capillary (75 microm x 8.5 cm) using a buffer of 30 mM Tris and 4 mM phosphoric acid at pH 8.9. Under the separation voltage of 3 kV, the quinolones were separated within 2.8 min with good linearity (r(2) >or= 0.985). The method was successfully applied in determining OFL in a pharmaceutical formulation. Also, a liquid-liquid extraction (LLE) method was developed and coupled to CE-PGD in determining quinolones that spiked in milk samples. With dichloromethane and hexane for enrichment and purification, the LLE recoveries of the four quinolones were in the range of 77-106%. The detection limits of the quinolones with LLE-CE-PGD were from 23 to 65 ng/mL. The proposed CE-PGD method was validated with an HPLC method, and the results indicated consistency between the two methods. |
| Databáze: | OpenAIRE |
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