Molecular basis of Cyperus difformis cross-resistance to ALS-inhibiting herbicides
| Autor: | Grigorios Diamantidis, Ilias G. Eleftherohorinos, Nikolaos S Kaloumenos, S. Ntoanidou, Panagiotis Madesis |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis Population Als gene 03 medical and health sciences Cyperus difformis Sequence Homology Nucleic Acid Splice junction Cyperus Enzyme Inhibitors education Cross-resistance Genetics chemistry.chemical_classification education.field_of_study biology Base Sequence Herbicides Point mutation Intron 04 agricultural and veterinary sciences General Medicine biology.organism_classification Molecular biology Introns Acetolactate Synthase 030104 developmental biology Enzyme chemistry 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Agronomy and Crop Science |
| Zdroj: | Pesticide biochemistry and physiology. 127 |
| ISSN: | 1095-9939 |
| Popis: | Applications of 64 times higher rates of azimsulfuron and halosulfuron-methyl failed to reduce by 50% growth of 10 putative resistant (R) Cyperus difformis (smallflower umbrella-sedge) populations collected from rice fields located in Northern Greece. However, the growth rate of the susceptible (S) population was reduced by 50% by 1/4 of the recommended rate of both herbicides. The als gene sequencing identified point mutations at Pro-197 position, leading to amino acid substitution by Ala or Ser of the ALS enzyme. The in vitro activity of the ALS enzyme indicated that the I 50 values (herbicide concentration required for 50% reduction of the ALS activity) ranged from 10.7 to 55.5 μM azimsulfuron and from 6.7 to 50.6 μM halosulfuron-methyl, whereas the respective values for the S population were 0.09 and 0.11 μM. These results strongly support that cross-resistance of 10 C. difformis populations was due to point mutations of the als gene, which resulted in a less-sensitive ALS enzyme. This study reports the presence of a 1240 bp intron in the C. difformis als gene with the Pro-197 point mutations near the splice junction. |
| Databáze: | OpenAIRE |
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