Evolution of an expanded mannose receptor gene family
| Autor: | Lawrence G. Hunt, C. Butter, John R. Young, Karen Staines |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cytoplasm
Xenopus lcsh:Medicine Antigen Processing and Recognition Genome Mass Spectrometry Zoonoses Lectins Gene duplication Gene cluster Genetics of the Immune System Receptors Immunologic lcsh:Science Phylogeny Genetics Multidisciplinary biology Lizards Veterinary Diseases Multigene Family C700 Molecular Biology Biophysics and Biochemistry Mannose Receptor Research Article Veterinary Medicine Evolutionary Immunology Immunology Molecular Sequence Data Receptors Cell Surface Locus (genetics) Veterinary Immunology Antibodies Birds Evolution Molecular Animal Influenza Species Specificity Animals Humans Gene family Lectins C-Type Amino Acid Sequence RNA Messenger Antigen Gene Gene Evolutionary Biology Sequence Homology Amino Acid Macrophages lcsh:R Biology and Life Sciences Computational Biology Comparative Genomics Veterinary Virology biology.organism_classification Protein Structure Tertiary Mannose-Binding Lectins RNA Clinical Immunology Veterinary Science lcsh:Q Peptides Painted turtle Chickens |
| Zdroj: | PLoS ONE, Vol 9, Iss 11, p e110330 (2014) PLoS ONE |
| Popis: | Sequences of peptides from a protein specifically immunoprecipitated by an antibody, KUL01, that recognises chicken macrophages, identified a homologue of the mammalian mannose receptor, MRC1, which we called MRC1L-B. Inspection of the genomic environment of the chicken gene revealed an array of five paralogous genes, MRC1L-A to MRC1L-E, located between conserved flanking genes found either side of the single MRC1 gene in mammals. Transcripts of all five genes were detected in RNA from a macrophage cell line and other RNAs, whose sequences allowed the precise definition of spliced exons, confirming or correcting existing bioinformatic annotation. The confirmed gene structures were used to locate orthologues of all five genes in the genomes of two other avian species and of the painted turtle, all with intact coding sequences. The lizard genome had only three genes, one orthologue of MRC1L-A and two orthologues of the MRC1L-B antigen gene resulting from a recent duplication. The Xenopus genome, like that of most mammals, had only a single MRC1-like gene at the corresponding locus. MRC1L-A and MRC1L-B genes had similar cytoplasmic regions that may be indicative of similar subcellular migration and functions. Cytoplasmic regions of the other three genes were very divergent, possibly indicating the evolution of a new functional repertoire for this family of molecules, which might include novel interactions with pathogens. |
| Databáze: | OpenAIRE |
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