Effect of Surfactants on Plasmid DNA Stability and Release from Poly (D,L-lactide-co-glycolide) Microspheres

Autor: Nur 'Izzati Mansor, Nurul Hafizah Mohd Nor, Ahmad Fahmi Harun Ismail, Farahidah Mohamed, Abd Almonem Doolaanea
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Tropical Journal of Pharmaceutical Research; Vol 14, No 10 (2015); 1769-1778
ISSN: 1596-5996
1596-9827
Popis: Purpose : To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods : Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the impact of different surfactants on pDNA encapsulation. Results : Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fractiondependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100 (HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100 exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate in DNA release. Conclusion : The microspheres were non-toxic to the neuro-2a cells which suggest they can be potentially used in the gene therapy of neuronal diseases. Keywords : Surfactant, Gene therapy, Microspheres, Polylactic glycolide, Plasmid DNA, Supercoil index, Hydrophilic-lipophilic balance
Databáze: OpenAIRE
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