Effects of pharmacologic sclerostin inhibition or testosterone administration on soleus muscle atrophy in rodents after spinal cord injury

Autor: Anna C F Bassit, Fan Ye, Dana M. Otzel, Luke A. Beggs, Payal Ghosh, Stephen E. Borst, Darren T. Beck, Joshua F. Yarrow, Ean G. Phillips, Christine F. Conover
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Critical Care and Emergency Medicine
lcsh:Medicine
Biochemistry
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Testosterone
Lipid Hormones
lcsh:Science
Spinal Cord Injury
Spinal cord injury
Musculoskeletal System
Trauma Medicine
Mammals
Multidisciplinary
Muscles
Eukaryota
Muscle Analysis
Muscular Atrophy
Bioassays and Physiological Analysis
Neurology
Receptors
Androgen
Vertebrates
Bone Morphogenetic Proteins
Androgens
Anatomy
Cellular Types
Traumatic Injury
Research Article
Genetic Markers
medicine.medical_specialty
medicine.drug_class
030209 endocrinology & metabolism
Slow-Twitch Muscle Fibers
Research and Analysis Methods
Rodents
Muscle Fibers
03 medical and health sciences
Atrophy
Internal medicine
medicine
Animals
Muscle
Skeletal
Spinal Cord Injuries
Soleus muscle
business.industry
lcsh:R
Organisms
Biology and Life Sciences
Soleus Muscles
Cell Biology
Skeletal Muscle Fibers
medicine.disease
Androgen
Antibodies
Neutralizing
Hormones
Rats
Androgen receptor
Endocrinology
Levator ani
chemistry
Skeletal Muscles
Amniotes
Sclerostin
lcsh:Q
business
Neurotrauma
030217 neurology & neurosurgery
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 3, p e0194440 (2018)
ISSN: 1932-6203
Popis: Sclerostin is a circulating osteocyte-derived glycoprotein that negatively regulates Wnt-signaling after binding the LRP5/LRP6 co-receptors. Pharmacologic sclerostin inhibition produces bone anabolic effects after spinal cord injury (SCI), however, the effects of sclerostin-antibody (Scl-Ab) on muscle morphology remain unknown. In comparison, androgen administration produces bone antiresorptive effects after SCI and some, but not all, studies have reported that testosterone treatment ameliorates skeletal muscle atrophy in this context. Our purposes were to determine whether Scl-Ab prevents hindlimb muscle loss after SCI and compare the effects of Scl-Ab to testosterone enanthate (TE), an agent with known myotrophic effects. Male Sprague-Dawley rats aged 5 months received: (A) SHAM surgery (T8 laminectomy), (B) moderate-severe contusion SCI, (C) SCI+TE (7.0 mg/wk, im), or (D) SCI+Scl-Ab (25 mg/kg, twice weekly, sc). Twenty-one days post-injury, SCI animals exhibited a 31% lower soleus mass in comparison to SHAM, accompanied by >50% lower soleus muscle fiber cross-sectional area (fCSA) (p
Databáze: OpenAIRE
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