Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2
| Autor: | John J. Worthington, Nadin Fathallah, Vinay Shivanna, Fatai S. Oladunni, Luis Martinez-Sobrido, Mohammed A. Rohaim, Lucy H. Jackson-Jones, Jun Gyu Park, Wafaa A. Alhazmi, Jordi B. Torrelles, Renee Escalona, Muhsref Bakri Assas, Pengxiang Chang, Muhammad Munir, Munir Iqbal, Matthew D. Hodges, Jayde Whittingham-Dowd, Abdullah Almilaibary, James Tollitt |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Immunoglobulin A
Science viruses Newcastle disease Virus Article immunology Immunity Medicine Vaccines Multidisciplinary Attenuated vaccine biology Pandemic business.industry SARS-CoV-2 Immunogenicity Viral Infection virus diseases COVID-19 respiratory system biochemical phenomena metabolism and nutrition biology.organism_classification Virology virology respiratory tract diseases Vaccination biology.protein Nasal administration business |
| Zdroj: | iScience iScience, Vol 24, Iss 9, Pp 102941-(2021) |
| ISSN: | 2589-0042 |
| Popis: | Global deployment of an effective and safe vaccine is necessary to curtail the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2. Intranasal administration of recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 to mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters immunised with two doses of vaccine showed complete protection from lung infection, inflammation, and pathological lesions following SARS-CoV-2 challenge. Importantly, administration of two doses of intranasal rNDV-S vaccine significantly reduced the SARS-CoV-2 shedding in nasal turbinate and lungs in hamsters. Collectively, intranasal vaccination has the potential to control infection at the site of inoculation, which should prevent both clinical disease and virus transmission to halt the spread of the COVID-19 pandemic. Graphical Abstract |
| Databáze: | OpenAIRE |
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