Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer
| Autor: | Tom Brown, Alastair Aitken, Ross Stevenson, Robert Baxter, H. C. Baxter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Gene isoform
Aptamer Molecular Sequence Data Oligonucleotides DNA Single-Stranded Scrapie Plasma protein binding Biochemistry Chromatography Affinity law.invention chemistry.chemical_compound law Drug Discovery Animals Humans Protein–DNA interaction Cloning Molecular Molecular Biology Sheep Base Sequence Organic Chemistry SELEX Aptamer Technique DNA Aptamers Nucleotide Molecular biology Recombinant Proteins chemistry 14-3-3 Proteins Recombinant DNA Protein Binding |
| Popis: | A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep. |
| Databáze: | OpenAIRE |
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