Incorporation of Fibrin Matrix into Electrospun Membranes for Periodontal Wound Healing
| Autor: | Suyog Yoganarasimha, Caroline K. Carrico, Parthasarathy Madurantakam, Choyi Wong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Bioengineering
wound healing 02 engineering and technology lcsh:Technology Article Fibrin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Centrifugation fibrin lcsh:QH301-705.5 electrospinning membranes regeneration GTR biology lcsh:T technology industry and agriculture 030206 dentistry 021001 nanoscience & nanotechnology Trypsin Electrospinning Membrane chemistry lcsh:Biology (General) Polycaprolactone biology.protein Genipin 0210 nano-technology Wound healing Biomedical engineering medicine.drug |
| Zdroj: | Bioengineering Volume 6 Issue 3 Bioengineering, Vol 6, Iss 3, p 57 (2019) Bioengineering, 6(3):57 |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering6030057 |
| Popis: | Guided tissue regeneration (GTR) aims to regenerate the lost attachment apparatus caused by periodontal disease through the use of a membrane. The goal of this study is to create and characterize a novel hybrid membrane that contains biologically active fibrin matrix within a synthetic polycaprolactone (PCL) electrospun membrane. Three-dimensional fibrin matrices and fibrin-incorporated electrospun membrane were created from fresh frozen plasma by centrifugation in glass vials under three different conditions: 400 g for 12 min, 1450 g for 15 min and 3000 g for 60 min. Half the membranes were crosslinked with 1% genipin. Degradation against trypsin indicated biologic stability while uniaxial tensile testing characterized mechanical properties. Continuous data was analyzed by ANOVA to detect differences between groups (p = 0.05). Fibrin-incorporated electrospun membranes showed statistically significant increase in mechanical properties (elastic modulus, strain at break and energy to break) compared to fibrin matrices. While crosslinking had marginal effects on mechanical properties, it did significantly increase biologic stability against trypsin (p < 0.0001). Lastly, membranes generated at 400 g and 1450 g were superior in mechanical properties and biologic stability compared to those generated at 3000 g. Fibrin-incorporated, crosslinked electrospun PCL membranes generated at lower centrifugation forces offers a novel strategy to generate a potentially superior membrane for GTR procedures. |
| Databáze: | OpenAIRE |
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