A SENSITIVE, PRECISE RADIOIMMUNOASSAY OF SERUM INSULIN RELYING ON CHARCOAL SEPARATION OF BOUND AND FREE HORMONE MOIETIES
| Autor: | J. D. M. Albano, G. Maritz, R. C. Turner, R. P. Ekins |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Serum insulin Radioimmunoassay Endocrinology Blood serum Iodine Isotopes Internal medicine Blood plasma Methods medicine Humans Insulin Free hormone Charcoal Chemistry General Medicine Hypoglycemia Diabetes Mellitus Type 1 visual_art visual_art.visual_art_medium Kidney Diseases Hormone |
| Zdroj: | Acta Endocrinologica. 70:487-509 |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/acta.0.0700487 |
| Popis: | A radioimmunoassay method for the measurement of plasma insulin is described relying on activated charcoal for the separation of free and bound fractions. The technique illustrates the application of theoretical precepts designed to maximise assay precision in all radioimmunoassay and other saturation assay techniques. In addition, because particular emphasis has been placed on ensuring that, as far as is possible, all incubation mixtures are similar as possible other than in hormone concentration, non-specific effects appear to have been essentially eliminated. The technique yields a mean normal fasting value of 5.3μU/ml (range 2–14 μU/ml). Its sensitivity is such that 10 μl samples of serum (or plasma) may be assayed. |
| Databáze: | OpenAIRE |
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