PAF-acether transfer activity in HL-60 cells is induced during differentiation
| Autor: | Gérard Ribbes, G.L. Pool, Hugues Chap, Michel Record, François Tercé, R.H. Lumb |
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| Rok vydání: | 1990 |
| Předmět: |
Cellular differentiation
Biophysics Receptors Cell Surface Platelet Membrane Glycoproteins Biology Biochemistry Cell Line Receptors G-Protein-Coupled Proinflammatory cytokine chemistry.chemical_compound Cytosol Leukemia Promyelocytic Acute Humans Cytotoxic T cell Dimethyl Sulfoxide Platelet Activating Factor Molecular Biology Platelet-activating factor Macrophages Cell Differentiation Biological activity Cell Biology Molecular biology Kinetics Bucladesine chemistry Cell culture Chromatography Gel Phorbol Tetradecanoylphorbol Acetate Specific activity Granulocytes |
| Zdroj: | Biochemical and Biophysical Research Communications. 171:548-554 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(90)91181-q |
| Popis: | Platelet-activating factor is a proinflammatory lipid active at subnanomolar concentrations. The intermembrane transfer of a biologically active PAF analog has been previously demonstrated in macrophages. Here we demonstrate that the specific activity of this transfer activity increases when HL-60 cells are induced to differentiate by treatment with dimethyl sulfoxide, dibutyryl cAMP or phorbol diester. In undifferentiated HL-60 cells, methylcarbamyl-PAF transfer activity was only 0.56 U·min −1 ·mg −1 . This basal value was increased 2.6 and 6.7 times upon granulocytic and macrophagic differentiation, respectively. On the other hand, the transfer of 2-O-methyl-PAF, a cytotoxic analog with no PAF biological activity, remained very low and did not vary during differentiation. |
| Databáze: | OpenAIRE |
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