Single-Cell Proteomics Defines the Cellular Heterogeneity of Localized Prostate Cancer
| Autor: | Wild, Peter J., De Vargas Roditi, Laura, Jacobs, Andrea, Rüschoff, Jan H., Bankhead, Pete, Chevrier, Stéphane, Jackson, Hartland W., Hermanns, Thomas, Fankhauser, Christian D., Poyet, Cedric, Chun, Felix, Rupp, Niels J., Tschaebunin, Alexandra, Bodenmiller, Bernd |
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| Přispěvatelé: | University of Zurich, Bodenmiller, Bernd, Wild, Peter Johannes |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics Cellular heterogeneity Stromal cell 610 Medicine & health 2700 General Medicine Disease Biology single-cell proteomics General Biochemistry Genetics and Molecular Biology Prostate cancer Immune system 1300 General Biochemistry Genetics and Molecular Biology 10049 Institute of Pathology and Molecular Pathology Tumor Microenvironment medicine Humans Mass cytometry Prostate Prostatic Neoplasms Genomics prostate cancer medicine.disease 10062 Urological Clinic Localized disease Cancer research Biomarker (medicine) Orchiectomy 11493 Department of Quantitative Biomedicine |
| Zdroj: | Cell Reports Medicine, 3 (4) De Vargas Roditi, L, Jacobs, A, Rueschoff, J H, Bankhead, P, Chevrier, S, Jackson, H W, Hermanns, T, Fankhauser, C D, Poyet, C, Chun, F, Rupp, N J, Tschaebunin, A, Bodenmiller, B & Wild, P J 2022, ' Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer ', Cell Reports Medicine, vol. 3, no. 4, pp. 100604 . https://doi.org/10.1016/j.xcrm.2022.100604 |
| ISSN: | 2666-3791 |
| Popis: | Localized prostate cancer exhibits multiple genomic alterations and heterogeneity at the proteomic level. Single-cell technologies capture important cell-to-cell variability responsible for heterogeneity in biomarker expression that may be overlooked when molecular alterations are based on bulk tissue samples. This study aims to identify prognostic biomarkers and describe the heterogeneity of prostate cancer and the associated microenvironment by simultaneously quantifying 36 proteins using single-cell mass cytometry analysis of over 1.6 million cells from 58 men with localized prostate cancer. We perform this task, using a high-dimensional clustering pipeline named Franken to describe subpopulations of immune, stromal, and prostate cells, including changes occurring in tumor tissues and high-grade disease that provide insights into the coordinated progression of prostate cancer. Our results further indicate that men with localized disease already harbor rare subpopulations that typically occur in castration-resistant and metastatic disease. Cell Reports Medicine, 3 (4) ISSN:2666-3791 |
| Databáze: | OpenAIRE |
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