Purification of Fibroblast-Derived Celiac Disease Autoantigen Molecules
| Autor: | Aulis Marttinen, Markku Mäki |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Immunofluorescence
Autoantigens Antibodies Chromatography Affinity Gliadin Pathogenesis Extracellular matrix Fetus medicine Humans Fibroblast Lung Cells Cultured biology medicine.diagnostic_test Fibroblasts Endomysium Molecular biology In vitro Immunoglobulin A Molecular Weight Celiac Disease medicine.anatomical_structure Pediatrics Perinatology and Child Health Immunology Chromatography Gel biology.protein Isoelectric Focusing Antibody |
| Zdroj: | Pediatric Research. 34:420-423 |
| ISSN: | 1530-0447 0031-3998 |
| Popis: | We have recently purified autoantigen polypeptides reacting with celiac disease patient sera IgA from the extracellular noncollagenous matrix compartment of fetal lung tissue. These molecules trigger the production of different tissue antibodies, the so-called antireticulin and antiendomysium antibodies in celiac disease. In the present report, we show that fibroblasts synthesize and secrete celiac disease autoantigen molecules. The secretion product, reactive with IgA from celiac disease patients, is a large-molecular-weight protein aggregate. When the protein complex was treated with 4 M guanidinium hydrochloride and 0.1% SDS, 11 monocomponent polypeptides could be detected by PAGE. Of these, four single polypeptides with molecular weights of 17 000–39 500 and isoelectric points of 5.0–7.0 were observed to react with IgA separated from sera of children with celiac disease. The polypeptide molecules produced by fibroblasts in vitro bound to antireticulin and antiendomysium antibodies but not to antigliadin antibodies. The present observations show that tissue antibodies found to be specifically associated with celiac disease are generated against a synthesis product of fibroblasts, a cell-type known to synthesize a number of biologically active polypeptides. The fibroblast-derived extracellular matrix proteins and the formed autoantibodies may be important in the pathogenesis of gluten-sensitive enteropathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|