LC-MS/MS determination of colistin in Mueller–Hinton broth for in vitro pharmacodynamic studies
| Autor: | Xiao-Jie Wu, Jian Li, Yu-Ran Cao, Jing Zhang, Bei-Ning Guo, Miao Zhao |
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| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
Spectrometry Mass Electrospray Ionization Analyte Chromatography Colistin Calibration curve Coefficient of variation Selected reaction monitoring Reproducibility of Results Bacterial growth Biology Tandem mass spectrometry Culture Media Tandem Mass Spectrometry Pharmacodynamics Drug Discovery Linear Models medicine Humans Chromatography Liquid medicine.drug |
| Zdroj: | The Journal of Antibiotics. 67:825-829 |
| ISSN: | 1881-1469 0021-8820 |
| Popis: | A rapid and simple method was developed and validated for the determination of colistin A and B in Mueller-Hinton broth using LC-tandem MS. Both analyte and internal standard (IS) (polymyxin B1) were determined using ESI. The MS data were obtained via the selected reaction monitoring in positive-ion mode. A linear regression (weighted 1/concentration) was used to fit calibration curves over the concentration range of 0.0241-2.41 μg ml(-1) for colistin A and 0.0439-4.39 μg ml(-1) for colistin B. No interference peaks were found in the blank Mueller-Hinton broth tested. Inter- and intraday precision and accuracy were within 85-115% (coefficient of variation). Colistin was stable in the autosampler for at least 24 h at 4 °C and in Mueller-Hinton broth for at least 120 h at 35 °C. This assay has been successfully used to determine colistin A and B in Mueller-Hinton broth for in vitro pharmacodynamic model studies. Accurate determination of colistin in bacterial growth medium has a vital role in the studies examining dosage regimen of and bacterial resistance to colistin. |
| Databáze: | OpenAIRE |
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