Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties

Autor: Wilfred A. Jefferies, Fumio Takei, Dixie L. Mager, Jack Brennan
Rok vydání: 1994
Předmět:
DNA
Complementary
Immunology
Molecular Sequence Data
CD1
Gene Expression
Mice
Inbred Strains
Major histocompatibility complex
Transfection
Polymerase Chain Reaction
Natural killer cell
Cell Line
Major Histocompatibility Complex
Mice
Antigen
MHC class I
Chlorocebus aethiops
Consensus Sequence
medicine
Cell Adhesion
Immunology and Allergy
Animals
Antigens
Ly
Humans
Lectins
C-Type
Amino Acid Sequence
Cloning
Molecular
Cell adhesion
Cells
Cultured
DNA Primers
COS cells
Membrane Glycoproteins
biology
Base Sequence
Sequence Homology
Amino Acid
Articles
MHC restriction
Molecular biology
Lymphocyte Subsets
Killer Cells
Natural
Mice
Inbred C57BL
medicine.anatomical_structure
Multigene Family
Antigens
Surface
biology.protein
NK Cell Lectin-Like Receptor Subfamily A
Spleen
Receptors
NK Cell Lectin-Like
Zdroj: The Journal of Experimental Medicine
ISSN: 0022-1007
Popis: The murine Ly-49 antigen belongs to a family of type II transmembrane molecules containing lectin-like domains. The original member of this family, Ly-49A, has been demonstrated to be expressed by a subpopulation of natural killer (NK) cells, bind certain class I major histocompatibility complexes (MHC), and act as a negative regulator of lytic activity. The expression patterns and functional activities of the other Ly-49s, however, is unknown. We extended the study of this family by isolating cDNAs encoding two new Ly-49 molecules. The reactivity of these and previously identified Ly-49 molecules with NK antibodies was tested in a COS cell expression system. YE1/32 and YE1/48 bound Ly-49A specifically, and 5E6 reacted only with Ly-49C. Three-color flow cytometric analysis demonstrated Ly-49A and Ly-49C expression defines complex, but distinct subsets within NK1.1+ cells. Some NK1.1-CD3+ as well as NK1.1-CD3- cells expressing Ly-49A or C were also detected. Analysis of MHC congenic strains of mice demonstrated that YE1/32+ and YE1/48+ NK cells are not deleted, as has been shown with the Ly-49A mAb A1. Furthermore, COS cells transfected with Ly-49A bound H-2d and H-2k cell lines, whereas Ly-49C transfectants bound H-2d, H-2k, H-2b, and H-2s. The antibodies 5E6 and 34-1-2S (anti-class I MHC) inhibited the binding of Ly-49C to an H-2s cell line. These results imply that the NK cell antigens Ly-49A and C bind to different repertoires of class I MHC molecules.
Databáze: OpenAIRE
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