A comparative clinical study on the generation of nitrosative stress in cataractous lenses of smokers and non-smoker tobacco patients
| Autor: | Krishnagopal Srikanth, Subrayan Suganya, Subramanian Muthukumar, Thirugnanasambandhar Sivasubramanian Anitha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Nitric Oxide Synthase Type II Enzyme-Linked Immunosorbent Assay Nitric Oxide Cataract Nitric oxide Clinical study Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Lens Crystalline medicine Humans Smokers biology business.industry Smoking General Medicine Non-Smokers Middle Aged Nitric oxide synthase Ophthalmology Chewing tobacco 030104 developmental biology Endocrinology chemistry Nitrosative Stress 030221 ophthalmology & optometry biology.protein Tyrosine Female business |
| Zdroj: | European journal of ophthalmology. 29(2) |
| ISSN: | 1724-6016 |
| Popis: | Aim: To quantify the levels of nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine in cataractous lenses of smokers and smokers who chewed tobacco in comparison with non-smokers and non-smokers who chewed tobacco. Study design: A total of 80 cataractous lenses from smokers, non-smokers, smokers with tobacco chewing habit, and non-smokers with tobacco chewing habit were collected from the patients who had enrolled in the Department of Ophthalmology, Mahatma Gandhi Medical College & Research Institute, Puducherry. Methods: Levels of nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine were quantified using commercially available enzyme-linked immunosorbent assay kits. Results: The mean concentrations of lens nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine are as follows: (a) smokers—112.01, 59.57, and 88.91 µmol/L; (b) smokers who chewed tobacco—175.15, 93.95, and 128.72 µmol/L; (c) non-smokers—76.15, 40.65, and 70.20 µmol/L; and (d) non-smokers who chewed tobacco—96.56, 52.87, and 83.88 µmol/L, respectively. Conclusion: Nitric oxide, inducible nitric oxide synthase, and 3-nitrotyrosine at high levels are the major causative agents for cataractogenesis. The results of this study suggest that smoking and tobacco chewing habit generate nitrosative stress that could enhance the pathogenesis for early cataractogenesis. |
| Databáze: | OpenAIRE |
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