Cellular response to orthodontically-induced short-term hypoxia in dental pulp cells
Autor: | Michael Wolf, Claudia Reicheneder, Jochen Fanghänel, Peter Proff, Piero Römer |
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Rok vydání: | 2013 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Histology Tooth Movement Techniques Dentistry medicine.disease_cause Pathology and Forensic Medicine Flow cytometry Young Adult stomatognathic system In vivo medicine Animals Humans Periodontal fiber Rats Wistar Cells Cultured Dental Pulp medicine.diagnostic_test business.industry Cell Biology Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit Molecular medicine Cell Hypoxia Rats Oxygen Oxidative Stress stomatognathic diseases Circulatory system Cytokines Pulp (tooth) medicine.symptom business Oxidative stress |
Zdroj: | Cell and Tissue Research. 355:173-180 |
ISSN: | 1432-0878 0302-766X |
Popis: | Orthodontic force application is well known to induce sterile inflammation, which is initially caused by the compression of blood vessels in tooth-supporting apparatus. The reaction of periodontal ligament cells to mechanical loading has been thoroughly investigated, whereas knowledge on tissue reactions of the dental pulp is rather limited. The aim of the present trial is to analyze the effect of orthodontic treatment on the induction and cellular regulation of intra-pulpal hypoxia. To investigate the effect of orthodontic force on dental pulp cells, which results in circulatory disturbances within the dental pulp, we used a rat model for the immunohistochemical analysis of the accumulation of hypoxia-inducible factor-1α in the initial phase of orthodontic tooth movement. To further examine the regulatory role of circulatory disturbances and hypoxic conditions, we analyze isolated dental pulp cells from human teeth with regard to their specific reaction under hypoxic conditions by means of flow cytometry, immunoblot, ELISA and real-time PCR on markers (Hif-1α, VEGF, Cox-2, IL-6, IL-8, ROS, p65). In vivo experiments showed the induction of hypoxia in dental pulp after orthodontic tooth movement. The induction of oxidative stress in human dental pulp cells showed up-regulation of the pro-inflammatory and angiogenic genes Cox-2, VEGF, IL-6 and IL-8. The present data suggest that orthodontic tooth movement affects dental pulp circulation by hypoxia, which leads to an inflammatory response inside treated teeth. Therefore, pulp tissue may be expected to undergo a remodeling process after tooth movement. |
Databáze: | OpenAIRE |
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