Expression and localization of Rdd proteins in Xenopus embryo
| Autor: | Mitsugu Maéno, Rie Tamaki, Yutaka Mashima, Jong-Chan Lim, Sayaka Kurihara |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Morpholino Xenopus Biology Cellular and Molecular Neuroscience Extracellular medicine Cysteine-rich Antibody Vascular cells Embryogenesis Embryo Cell Biology Embryonic Tissue biology.organism_classification Development/Regeneration Cell biology Secretory protein Original Article Anatomy Vascular endothelial growth factor Immunostaining Frog Developmental Biology |
| Zdroj: | Anatomy & Cell Biology |
| ISSN: | 2093-3665 |
| Popis: | The previous study has shown that repeated D domain-like (Rdd) proteins, a group of novel secretory proteins consisting of repeated domains of a cysteine-rich sequence, are involved in the process of blood vessel formation in Xenopus embryo. We performed further experiments to examine the localization of Rdd proteins in embryogenesis. Detection of tagged Rdd proteins expressed in blastomeres showed that Rdd proteins formed a high molecular weight complex and existed in the extracellular space. A rabbit antibody against the Rdd synthetic peptide identified a single band of 28 kD in embryonic tissue extract. By whole-mount immunostaining analysis, signal was detected in the regions of inter-somites, vitelline veins, and branchial arches at the tailbud stage. Staining of Rdd was remarkably reduced in the embryos injected with vascular endothelial growth factor Morpholino. We suggest that Rdd proteins interact with a molecule(s) associated with vascular precursor cells. |
| Databáze: | OpenAIRE |
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