Long-term impact of fecal transplantation in healthy volunteers
| Autor: | Vera Pawlowsky-Glahn, Vladimir A. Veselovsky, Vadim M. Govorun, Juan José Egozcue, Elena S. Kostryukova, Alexander I. Manolov, Ivan S. Moiseev, Margarita V. Gorchakova, Alexander A. Shcherbakov, Alexey B. Chukhlovin, Vladimir Gostev, Evgenii I. Olekhnovich, Alexander N. Shvetcov, Maxim A. Kucher, Alexander V. Pavlenko, Elvira D. Gumbatova, O. Goloshchapov, Elena N. Ilina, Evgeny A. Bakin, Ruslana V. Klementeva, Boris V. Afanasyev, Sergey Sidorenko, Dmitry E. Fedorov, Ksenia M. Klimina, Maria A. Suvorova |
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| Přispěvatelé: | Agencia Estatal de Investigación, Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Time Factors lcsh:QR1-502 Metagenome-assembled genome Disease Gut flora Gastroenterology lcsh:Microbiology Fecal microbiota transplantation Excrements -- Microbiologia Taxonomic composition Feces fluids and secretions 0302 clinical medicine RNA Ribosomal 16S Healthy volunteers Feces--Microbiology Volunteer Estimation theory 16S rRNA gene sequencing 0303 health sciences Compositional data analysis Fecal Microbiota Transplantation Middle Aged Ulcerative colitis Healthy Volunteers Intestins -- Microbiologia 030211 gastroenterology & hepatology Female Research Article Microbiology (medical) Adult medicine.medical_specialty Ciències de la salut::Medicina::Medicina interna [Àrees temàtiques de la UPC] Biology Intestines -- Microbiology Microbiology digestive system 03 medical and health sciences Immune system Internal medicine medicine Humans Adverse effect Estimació Teoria de l' 030304 developmental biology business.industry Fecal bacteriotherapy biology.organism_classification medicine.disease Shotgun sequencing Gastrointestinal Microbiome Systemic inflammatory response syndrome Immunology Metagenomics business |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 19, Iss 1, Pp 1-13 (2019) UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) BMC Microbiology, 2019, vol. 19, art. núm. 312 Articles publicats (D-IMA) DUGiDocs – Universitat de Girona instname |
| DOI: | 10.1101/671644 |
| Popis: | Background Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in the majority of studies the effects of FMT were evaluated on the patients with initially altered microbiota. The aim of our study was to estimate effects of FMT on the gut microbiota composition in healthy volunteers and to monitor its long-term outcomes. Results We have performed a combined analysis of three healthy volunteers before and after capsule FMT by evaluating their general condition, adverse clinical effects, changes of basic laboratory parameters, and several immune markers. Intestinal microbiota samples were evaluated by 16S rRNA gene and shotgun sequencing. The data analysis demonstrated profound shift towards the donor microbiota taxonomic composition in all volunteers. Following FMT, all the volunteers exhibited gut colonization with donor gut bacteria and persistence of this effect for almost ∼1 year of observation. Transient changes of immune parameters were consistent with suppression of T-cell cytotoxicity. FMT was well tolerated with mild gastrointestinal adverse events, however, one volunteer developed a systemic inflammatory response syndrome. Conclusions The FMT leads to significant long-term changes of the gut microbiota in healthy volunteers with the shift towards donor microbiota composition and represents a relatively safe procedure to the recipients without long-term adverse events. |
| Databáze: | OpenAIRE |
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