Effects of vinblastine on calcium distribution pattern and Ca2+,Mg(2+)-adenosine triphosphatase in rat incisor maturation ameloblasts
| Autor: | A. H. Salama, A. M. E. Zaki, D. R. Eisenmann |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Antimony
medicine.medical_specialty Histology ATPase chemistry.chemical_element Calcium-Transporting ATPases Calcium Vinblastine stomatognathic system Internal medicine medicine Ameloblasts Animals Ca(2+) Mg(2+)-ATPase Membranes biology Histocytochemistry Rats Inbred Strains Rats Incisor Microscopy Electron Endocrinology chemistry biology.protein Cytochemistry Ultrastructure Anatomy Ameloblast medicine.drug |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 40(1) |
| ISSN: | 0022-1554 |
| Popis: | Vinblastine is known to affect secretory and transport functions of ameloblasts. The effects of vinblastine on distribution patterns of membrane-associated calcium and Ca2+,Mg(2+)-ATPase in maturation ameloblasts were investigated cytochemically. The potassium pyroantimonate (PPA) method was used for localizing calcium and a modified Wachstein-Meisel medium was used to localize Ca2+,Mg(2+)-ATPase. Ultrastructural changes induced by vinblastine included dislocated organelles and reduction or elimination of the ruffled border of the ameloblasts. Membrane-associated calcium pyroantimonate deposits were markedly reduced. The intensity of Ca2+,Mg(2+)-ATPase reaction product was also markedly reduced by vinblastine. Concomitant reduction of membrane-associated calcium and Ca2+,Mg(2+)-ATPase lends support to a role for maturation ameloblasts in control of a cyclic pattern of influx of calcium to mineralizing enamel. |
| Databáze: | OpenAIRE |
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