Redox properties and cytoprotective actions of atranorin, a lichen secondary metabolite
| Autor: | João Paulo Almeida dos Santos, Matheus Augusto de Bittencourt Pasquali, José Cláudio Fonseca Moreira, Francilene Amaral da Silva, Ricardo Fagundes da Rocha, Mairim Russo Serafini, Thallita Kelly Rabelo, Fernanda Freitas Caregnato, Lucindo Quintans, Adriano Antunes de Souza Araújo, Daniel Pens Gelain, Marcelia Garcez Dória de Melo |
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| Rok vydání: | 2011 |
| Předmět: |
Antioxidant
Radical medicine.medical_treatment Cytotoxicity Free radicals medicine.disease_cause Toxicology Antioxidants Nitric oxide chemistry.chemical_compound Hydroxybenzoates medicine Humans MTT assay Hydrogen peroxide Cells Cultured Atranorin Superoxide Lichen metabolites Hydrogen Peroxide General Medicine chemistry Biochemistry Cytoprotection Oxidative stress Hydroxyl radical Oxidation-Reduction |
| Zdroj: | Toxicology in Vitro. 25(2):462-468 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2010.11.014 |
| Popis: | Atranorin (ATR) is a lichenic secondary metabolite with potential uses in pharmacology. Antinociceptive and antiinflammatory actions have been reported, and the use of atranorin-enriched lichen extracts in folk medicine is widespread. Nonetheless, very few data on ATR biological actions are available. Here, we evaluated free radical scavenging activities and antioxidant potential of ATR using various in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. Besides, we determined the cytoprotective effect of ATR on H(2)O(2)-challenged SH-SY5Y cells by the MTT assay. ATR exerts differential effects towards reactive species production, enhancing hydrogen peroxide and nitric oxide production and acting as a superoxide scavenger; no activity toward hydroxyl radical production/scavenging was observed. Besides, TRAP/TAR analysis indicated that atranorin acts as a general antioxidant, although it demonstrated to enhance peroxyl radical-induced lipoperoxidation in vitro. ATR was not cytotoxic, and also protected SH-SY5Y cells against H(2)O(2)-induced cell viability impairment. Our results suggest that ATR has a relevant redox-active action, acting as a pro-oxidant or antioxidant agent depending on the radical. Also, it will exert cytoprotective effects on cells under oxidative stress induced by H(2)O(2). |
| Databáze: | OpenAIRE |
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