Red blood cells imaging and antigen–antibody interaction measurement
| Autor: | Ahmed Touhami, Oussama Ouerghi, C Fretigny, Ali Othmane, H. Ben Ouada, N Jaffrezic-Renault |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Immunoassay
Erythrocytes biology Cell adhesion molecule Chemistry Atomic force microscopy Bioengineering Antigen-Antibody Complex Adhesion Microscopy Atomic Force ABO Blood-Group System Cell biology Antigen-Antibody Reactions Antigen-antibody interaction Red blood cell medicine.anatomical_structure Antigen medicine biology.protein Humans Molecule Binding Sites Antibody Antibody Molecular Biology Biotechnology |
| Zdroj: | Biomolecular Engineering. 19:189-193 |
| ISSN: | 1389-0344 |
| DOI: | 10.1016/s1389-0344(02)00045-x |
| Popis: | In the present study the atomic force microscope (AFM) was used to image the surface morphology of red blood cells (RBC) for the first time. The AFM yielded very reproducible images without appreciable modifications of the sample surfaces. In addition to this topographical imaging, we have developed an experimental approach to measure the binding strength between antibody (anti-A), and the RBC antigen A, when reversible bonds between specific molecules such as antigen and antibody mediate the adhesion. The experimental results suggest that the procedure established here may be used for specific antibody detection. This study has also enhanced our understanding under physiological conditions of molecular interaction in particular antigen–antibody. |
| Databáze: | OpenAIRE |
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