The neuroendocrine protein VGF is sorted into dense-core granules and is secreted apically by polarized rat thyroid epithelial cells

Autor: Roberta Possenti, Claudia Puri, Patrizia Rosa, Carlo Tacchetti, Andrea Levi, Lucio Nitsch, Flaviana Gentile, Annunziata Corteggio, Gaetano Calì, Chiara Zurzolo, Federico Calegari
Přispěvatelé: Istituto di Endocrinologia ed Oncologia Sperimentale, Consiglio Nazionale delle Ricerche [Roma] (CNR), Trafic membranaire et Pathogénèse, Institut Pasteur [Paris], Dipartimento di Biologia e Patologia Cellulare e Moleculare, Università degli studi di Napoli Federico II, Istituto di Neuroscience, INMM, Department of Neuroscience, University of Roma, Departimenta di Medicina Sperimentale, University of Genoa (UNIGE), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Institut Pasteur [Paris] (IP), University of Naples Federico II = Università degli studi di Napoli Federico II, Università degli studi di Genova = University of Genoa (UniGe), Gentile, F, Cali, G, Zurzolo, Chiara, Corteggio, Annunziata, Rosa, P, Calegari, F, Levi, A, Possenti, R, Puri, C, Tacchetti, C, Nitsch, Lucio, Gentile, F., Cali', G., Zurzolo, C., Corteggio, A., Rosa, P., Calegari, F., Levi, A., Possenti, R., Puri, C., Tacchetti, Carlo, Nitsch, L.
Rok vydání: 2004
Předmět:
Thyroid Gland
8-Bromo Cyclic Adenosine Monophosphate
MESH: Neuropeptides
medicine.disease_cause
Protein sorting
MESH: Recombinant Proteins
0302 clinical medicine
MESH: Bucladesine
MESH: Microscopy
Immunoelectron
MESH: 8-Bromo Cyclic Adenosine Monophosphate
Protein targeting
Cell polarity
Cyclic AMP
MESH: Animals
MESH: Proteins
Microscopy
Immunoelectron
MESH: Cyclic AMP
MESH: Mutagenesis
0303 health sciences
VGF
Cell Polarity
Chromogranin A
thyroid cells
MESH: Rats
Inbred Strains
Recombinant Proteins
Regulated secretion
Cell biology
Transport protein
Protein Transport
MESH: Thionucleotides
MESH: Epithelial Cells
Tetradecanoylphorbol Acetate
MESH: Cell Polarity
MESH: Protein Transport
endocrine system
MESH: Rats
Immunoelectron microscopy
MESH: Cytoplasmic Granules
Biology
Cytoplasmic Granules
Transfection
03 medical and health sciences
medicine
Animals
Secretion
MESH: Tetradecanoylphorbol Acetate
030304 developmental biology
Dense core granule
MESH: Transfection
Neuropeptides
Proteins
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Epithelial Cells
Rats
Inbred Strains
Cell Biology
Thionucleotides
Molecular biology
MESH: Thyroid Gland
Rats
Secretory protein
Bucladesine
MESH: Gene Deletion
Mutagenesis
biology.protein
Gene Deletion
030217 neurology & neurosurgery
Zdroj: Experimental cell research (2004).
info:cnr-pdr/source/autori:Gentile, F; Cali, G; Zurzolo, C; Corteggio, A; Rosa, P; Calegari, F; Levi, A; Possenti, R; Puri, C; Tacchetti, C; Nitsch, L/titolo:The neuroendocrine protein VGF is sorted into dense-core granules and is secreted apically by polarized rat thyroid epithelial cells/doi:/rivista:Experimental cell research/anno:2004/pagina_da:/pagina_a:/intervallo_pagine:/volume
Experimental Cell Research
Experimental Cell Research, Elsevier, 2004, 295 (1), pp.269-80. ⟨10.1016/j.yexcr.2004.01.010⟩
Experimental Cell Research, 2004, 295 (1), pp.269-80. ⟨10.1016/j.yexcr.2004.01.010⟩
Experimental cell research 295 (2004): 269–280. doi:10.1016/j.yexcr.2004.01.010
info:cnr-pdr/source/autori:Gentile F, Calì G, Zurzolo C, Corteggio A, Rosa P, Calegari F, Levi A, Possenti R, Puri C, Tacchetti C, Nitsch L./titolo:The neuroendocrine protein VGF is sorted into dense-core granules and is secreted apically by polarized rat thyroid epithelial cells./doi:10.1016%2Fj.yexcr.2004.01.010/rivista:Experimental cell research/anno:2004/pagina_da:269/pagina_a:280/intervallo_pagine:269–280/volume:295
ISSN: 0014-4827
1090-2422
Popis: We have expressed the neuroendocrine VGF protein in FRT rat thyroid cells to study the molecular mechanisms of its sorting to the regulated and polarized pathways of secretion. By immunoelectron microscopy, we have demonstrated that VGF localizes in dense-core granules. Rapid secretion of VGF is induced by PMA stimulation. Moreover, human chromogranin B, a protein of the regulated pathway, co-localizes in the same granules with VGF. In confluent, FRT monolayers on filters protein secretion occur from the apical cell domain. VGF deletion mutants have been generated. By confocal microscopy, we have found that in transient transfection, all mutant proteins are sorted into granules and co-localize with the full-length VGF. They all retain the apical polarity of secretion. We also found that intracellular VGF and its deletion mutants are largely in an aggregated form. We conclude that FRT thyroid cells correctly decode the sorting information of VGF. The signals present on the protein to enter the granules and to be secreted apically cannot be separated from each other and are not in just one discrete portion of the protein. We propose that selective aggregation might represent the signal for sorting VGF to the regulated, apical route.
Databáze: OpenAIRE
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