Biodegradation of chrysene and benzo[a]pyrene and removal of metals from naturally contaminated soil by isolated Trametes versicolor strain and laccase produced thereof
| Autor: | Ziva Vipotnik, Michele Michelin, Teresa Tavares |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2022 |
| Předmět: |
fungal degradation
polyaromatic hydrocarbons udc:54 Science & Technology Fungal degradation kemija razgradnja Soil Science onesnaževanje Plant Science metals removal kemija onesnaževanje lakaze razgradnja Metals removal lakaze contaminated soil Enzymatic degradation enzymatic degradation contaminated soil fungal degradation enzymatic degradation polyaromatic hydrocarbons metals removal Contaminated soil Polyaromatic hydrocarbons General Environmental Science |
| Zdroj: | Environmental technology & innovation, str. 1-12 : Ilustr., Vol. 28, [article no.] 102737, Nov. 2022 COBISS-ID: 522921753 Environmental technology & innovation, vol. 28, no. 102737, pp. 1-12, 2022. Environmental technology & innovation |
| ISSN: | 2352-1864 |
| DOI: | 10.1016/j.eti.2022.102737 |
| Popis: | "Available online 11 June 2022" The objective of this study was to assess the degradation rates of chrysene and benzo[a]pyrene, as well as the removal of aluminium and iron from contaminated soil collected in the upper layer (030 cm) in Lagos, Southwest Nigeria. Trametes versicolor was isolated from this soil and used in degradation experiments, with plantain peels as support. After 8 weeks, 82.0% of chrysene degradation was achieved by T. versicolor, and by adding support this increased to 91.0%. Benzo[a]pyrene was less degradable, with 38.0% and 49.1% of degradation, respectively. Trametes versicolor was also capable of accumulate 46.1% of aluminium and 57.2% of iron. By adding plantain peels, these amounts increased to 48.2% and 61.8%, respectively. At the same time, laccase was produced by Trametes versicolor on plantain peels, achieving 37.8 U/g of crude laccase during SSF at 30 °C for 3 weeks. Laccase degradation experiments were set up in packed-bed reactor (PBR), with a constant feed of 21.6 mL/day of laccase, with and without mediators. In 35 days, 75.9% degradation of chrysene was achieved by laccase. The highest degradation was observed with ABTS (2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt) as mediator, 87.9%. Benzo[a]pyrene degradation with laccase reached 35.6%, raising to 38.8 % with ferulic acid as mediator. In addition, 99.2% of iron and 99.6% of aluminium was removed by laccase, being the treatment for this last mediated with ABTS. This study was supported by the Portuguese Foundation for Science and Technology (FCT), Portugal under the scope of the research project PTDC/AAG-TEC/5269/2014, the strategic funding of UID/BIO/04469/2013 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Ziva Vipotnik is a recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) - Doctoral Program in Applied and Environmental Microbiology (DP_AEM); operation NORTE-08-5369-FSE-000060; co-financed by North 2020 through the European Social Fund (ESF). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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