Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs

Autor: J. Carlos Rodríguez-Cabello, Martijn van Griensven, Paulo C. Bessa, Asmita Banerjee, Raul Machado, António M. Cunha, Rui L. Reis, Sylvia Nürnberger, Heinz Redl, Daniela Dopler, Margarida Casal
Přispěvatelé: Universidade do Minho
Rok vydání: 2009
Předmět:
Drug Carriers/chemistry
Pharmaceutical Science
Nanoparticle
Bone Morphogenetic Protein 2
02 engineering and technology
Tissue engineering
Models
Growth Differentiation Factor 5
BMP-2
Cricetinae
Thermoresponsive
0303 health sciences
Drug Carriers
biology
Chemistry
Temperature
Biomaterial
Cell Differentiation
Anatomy
Elastin/chemistry
021001 nanoscience & nanotechnology
Bone Morphogenetic Protein 2/administration & dosage
Recombinant Proteins
3. Good health
Elastin-like polymer
Nanoparticles/chemistry
0210 nano-technology
C2C12
Delivery
Oligopeptides
Cell Survival/drug effects
Oligopeptides/chemistry
Cell Survival
Drug Compounding
Chemical
CHO Cells
Growth Differentiation Factor 5/administration & dosage
Bone morphogenetic protein
Bone morphogenetic protein 2
BMP-14
03 medical and health sciences
Cricetulus
Animals
Humans
Microparticle
Particle Size
030304 developmental biology
Science & Technology
Osteoblasts
Osteoblasts/cytology
Alkaline Phosphatase
Elastin
Freeze Drying
Models
Chemical
Solubility
Alkaline Phosphatase/metabolism
Cell Differentiation/drug effects
biology.protein
Biophysics
Nanoparticles
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 1873-4995
Popis: Elastin-like polymers are a new type of protein-based polymers that display interesting properties in the biomaterial field. Bone morphogenetic proteins (BMPs) are cytokines with a strong ability to promote new bone formation. In this work, we explored the use of elastin-like nanoparticles (average size 237.5±3.0 nm), created by thermoresponsive self-assembly, for the combined release of bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-14 (BMP-14). These BMPs could be encapsulated at high efficiency into the elastin-like particles and delivered in a sustained way for 14 days. The activity of the growth factors was retained, as shown by the induction of ALP activity and osteogenic mineralization in C2C12 cells. Increased bioactivity was observed with a combined release of BMP-2 and BMP-14. This approach shows a significant potential for future tissue engineering applications in bone.
This work was supported by Fundacao para a Ciencia e Tecnologia (PhD grants SFRH/BD/17049/2004 and SFRH/BD/36754/2007), project ElastM POCI/CTM/57177/2004 funded by FEDER and Fundacao para a Ciencia e Tecnologia; Marie Curie Alea Jacta EST short-term grant (MEST-CT-2004-8104) and European STREP Project HIPPOCRATES (NMP3-CT-2003-505758). This work was carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283). We acknowledge financial support from the MICINN (projects MAT 2007-66275-C02-01, NAN2004-08538, and PSE-300100-2006-1), the JCyL (projects VA034A09, VA016B08, and VA030A08), the CIBER-BBN (project CB06-01-0003), the JCyL and the Instituto de Salud Carlos III under the "Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon".
Databáze: OpenAIRE
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