| Autor: |
Sleijffers, A., Garssen, J., de Gruijl, F., Boland, G.J., van Hattum, J., van Vloten, W.A., van Loveren, H. |
| Přispěvatelé: |
Gezondheidsrisico Analyse en Toxicologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Photochemistry and Photobiology, 75(5), 541-546. Wiley-Blackwell |
| ISSN: |
0031-8655 |
| DOI: |
10.1562/0031-8655(2002)0750541ueiira2.0.co2 |
| Popis: |
UVB exposure impairs immune responses after hepatitis B vaccination in two different mouse strains. Sleijffers A, Garssen J, de Gruijl FR, Boland GJ, van Hattum J, van Vloten WA, van Loveren H. National Institute of Public Health and the Environment, Bilthoven, The Netherlands. annemarie.sleijffers@rivm.nl Ultraviolet light exposure can impair immune responses that are not restricted to the exposed skin but is also found at other sites, i.e. systemic immunosuppression. Therefore, we investigated the UV-induced modulating effects on vaccination against hepatitis B in a mouse model. Two different mouse strains, BALB/c and C57B1/ 6, were vaccinated intramuscularly against hepatitis B. Mice were exposed to different doses of ultraviolet B (UVB) for five consecutive days on shaved back skin before the vaccination. Vaccination against hepatitis B induced cellular (delayed-type hypersensitivity [DTH] and lymphocyte stimulation test) as well as humoral immune responses in both mouse strains. The DTH responses in C57BB1/6 mice were statistically significantly higher compared with BALB/c mice. UVB exposure induced a dose-dependent suppression of cellular immunity in both strains of mice. C57B1/6 mice seemed to be more susceptible to this suppression. Anti-hepatitis B surface antibodies (total-Ig) were only marginally suppressed after UVB exposure. IgG2a and interferon-gamma levels, both indicators for Th1 immune response, were suppressed in both mouse strains after UVB exposure. In summary, UVB exposure induced a dose-dependent suppression of both cellular and humoral immune responses after hepatitis B vaccination, although the suppressive effects on humoral immunity were limited to IgG2a production. Susceptibility to UVB-induced immunomodulation depended on the strain of mice and their predilection for developing different T cell responses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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