LC-MS/MS determination of ginsenoside compound K and its metabolite 20 (S)-protopanaxadiol in human plasma and urine: applications in a clinical study
| Autor: | Chun-Yang Wang, Yao Chen, Zhi-Rong Tan, Yicheng Wang, Li Yang, Wei-Hua Huang, Jingbo Peng, Xiao-Nv Xie, Dong-Sheng Ouyang |
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| Rok vydání: | 2019 |
| Předmět: |
Drug
Male Sapogenins Ginsenosides media_common.quotation_subject Metabolite Clinical Biochemistry Panax Urine Sapogenin Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry Arthritis Rheumatoid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry medicine Humans General Pharmacology Toxicology and Pharmaceutics media_common Chromatography Chemistry 010401 analytical chemistry General Medicine medicine.disease 0104 chemical sciences Medical Laboratory Technology Rheumatoid arthritis Protopanaxadiol Female Chromatography Liquid |
| Zdroj: | Bioanalysis. 11(5) |
| ISSN: | 1757-6199 |
| Popis: | Aim: Ginsenoside compound K (CK) is considered to be a potential therapeutic drug for rheumatoid arthritis because of its good anti-inflammatory activity. The purpose of this work was to establish a rapid, sensitive and specific method for determination of CK and its active metabolite 20(S)-protopanaxadiol (20(S)-PPD). Materials & methods: The analytes and internal standards were extracted by liquid–liquid extraction. Then, were separated by high performance liquid phase and determined by triple quadrupole mass spectrometry. Results: A LC–MS/MS using liquid–liquid extraction was developed for determining CK over the concentration range 1.00–1002.00 ng/ml and 0.15–54.30 ng/ml for 20(S)-PPD. The lower limits of quantification for CK and 20(S)-PPD were 1.00 and 0.15 ng/ml, respectively. Conclusion: This method was successfully validated for detecting both CK and 20(S)-PPD in the human plasma and urine, and was proved to be suitable for the pharmacokinetic study of CK in healthy Chinese volunteers. Clinical trial registration number: ChiCTR-TRC-14004824. |
| Databáze: | OpenAIRE |
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