Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution
Autor: | Matthew P. Davis, Alena Shkumatava, Tania Auchynnikava, Giovanni Bussotti, Harpreet K Saini, Juri Rappsilber, Jack M. Monahan, Claudia Carrieri, Anton J. Enright, Stijn van Dongen, Angelo Bitetti, Tommaso Leonardi, Robin C. Allshire, Dónal O'Carroll |
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Přispěvatelé: | Rappsilber, Juri [0000-0001-5999-1310], O'Carroll, Dónal [0000-0002-8626-2217], Enright, Anton J [0000-0002-6090-3100], Apollo - University of Cambridge Repository |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Transposable element Male Genome evolution Transcription Genetic Endogenous retrovirus Biology genome evolution Chromatin Epigenetics Genomics & Functional Genomics Genome Biochemistry Article Evolution Molecular 03 medical and health sciences Mice Open Reading Frames lncRNA Spermatocytes ddc:570 Genetics Animals Endogenous retroviruses Spermatogenesis Promoter Regions Genetic Gene Molecular Biology Endogenous Retroviruses Terminal Repeat Sequences Promoter Articles Genomics Long terminal repeat LncRNA spermatogenesis Chromatin 030104 developmental biology DNA Transposable Elements RNA Long Noncoding Transcriptome Transcription |
Zdroj: | Davis, M P, Carrieri, C, Saini, H K, van Dongen, S, Leonardi, T, Bussotti, G, Monahan, J M, Auchynnikava, T, Bitetti, A, Rappsilber, J, Allshire, R C, Shkumatava, A, O'Carroll, D & Enright, A J 2017, ' Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution ', EMBO Reports . https://doi.org/10.15252/embr.201744059 EMBO Reports |
Popis: | Spermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non‐coding transcripts (lncRNA). This process occurs post‐mitotically predominantly in spermatocytes and round spermatids. We demonstrate that this transposon‐driven lncRNA expression is a conserved feature of vertebrate spermatogenesis. We propose that transposon promoters are a mechanism by which the genome can explore novel transcriptional substrates, increasing evolutionary plasticity and allowing for the genesis of novel coding and non‐coding genes. Accordingly, we show that a small fraction of these novel ERV‐driven transcripts encode short open reading frames that produce detectable peptides. Finally, we find that distinct ERV elements from the same subfamilies act as differentially activated promoters in a tissue‐specific context. In summary, we demonstrate that LTRs can act as tissue‐specific promoters and contribute to post‐mitotic spermatogenic transcriptome diversity. |
Databáze: | OpenAIRE |
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