Limonia acidissima L. leaf mediated synthesis of zinc oxide nanoparticles: A potent tool against Mycobacterium tuberculosis
| Autor: | Bheemanagouda N. Patil, Tarikere C. Taranath |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Microbiology (medical)
lcsh:QR1-502 chemistry.chemical_element Nanoparticle 02 engineering and technology Zinc 010402 general chemistry Limonia acidissima 01 natural sciences lcsh:Microbiology Mycolic acid Mycobacterium tuberculosis Absorbance Limonia acidissima L Spectrophotometry Spectroscopy Fourier Transform Infrared Botany medicine Particle Size Fourier transform infrared spectroscopy Rutaceae chemistry.chemical_classification medicine.diagnostic_test biology Plant Extracts MABA Green Chemistry Technology 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Plant Leaves Infectious Diseases chemistry Nanoparticles Zinc oxide nanoparticles Zinc Oxide 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Mycobacteriology, Vol 5, Iss 2, Pp 197-204 (2016) |
| ISSN: | 2212-5531 |
| DOI: | 10.1016/j.ijmyco.2016.03.004 |
| Popis: | Objective/background The present investigation was undertaken to synthesize zinc oxide nanoparticles using Limonia acidissima L. and to test their efficacy against the growth of Mycobacterium tuberculosis. Methods The formation of zinc oxide nanoparticles was confirmed with UV–visible spectrophotometry. Fourier transform infrared spectroscopy shows the presence of bio-molecules involved in the stabilization of zinc oxide nanoparticles. The shape and size was confirmed with atomic force microscope, X-ray diffraction, and high resolution transmission electron microscope. These nanoparticles were tested for their effect on the growth of M. tuberculosis through the microplate alamar blue assay technique. Results The UV–visible data reveal that an absorbance peak at 374 nm confirms formation of zinc oxide nanoparticles and they are spherical in shape with sizes between 12 nm and 53 nm. These nanoparticles control the growth of M. tuberculosis at 12.5 μg/mL. Conclusion Phytosynthesis of zinc oxide nanoparticles is a green, eco-friendly technology because it is inexpensive and pollution free. In the present investigation, based on our results we conclude that the aqueous extract of leaves of L. acidissima can be used for the synthesis of zinc oxide nanoparticles. These nanoparticles control the growth of M. tuberculosis and this was confirmed with the microplate alamar blue method. The potential of biogenic zinc oxide nanoparticles may be harnessed as a novel medicine ingredient to combat tuberculosis disease. |
| Databáze: | OpenAIRE |
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