Changes in glycosidase activities during galactoglucomannan oligosaccharide inhibition of auxin induced growth
| Autor: | Peter Capek, Jozef Vojtaššák, Desana Lišková, Karin Kollárová, L. Bilisics |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Mannosidase
Glycoside Hydrolases Oligosaccharides Plant Science Horticulture Biology Biochemistry Mannans Cell wall chemistry.chemical_compound Cell Wall Extracellular Glycoside hydrolase Galactoglucomannan Molecular Biology chemistry.chemical_classification Indoleacetic Acids Plant Stems Peas food and beverages General Medicine Oligosaccharide Enzyme chemistry Intracellular |
| Zdroj: | Phytochemistry. 65:1903-1909 |
| ISSN: | 0031-9422 |
| Popis: | The inhibition of 2,4-D-induced elongation growth by galactoglucomannan oligosaccharides (GGMOs) in pea stem segments ( Pisum sativum L. cv. Tyrkys) after 18 h of incubation results in changes of extracellular, intracellular and cell wall glycosidase activities (β- d -glucosidase, β- d -mannosidase, β- d -galactosidase, β- d -xylosidase, α- d -galactosidase, and α- l -arabinosidase). GGMOs lowered the glycosidase activities in the extracellular fraction, while in the cell wall fractions their activities were markedly increased. The intracellular enzyme α- d -galactosidase increased while the β- d -galactosidase decreased in activity in response to the GGMO treatment. Extracellular enzymes showed low values of activities in comparison with intracellular and cell wall glycosidases. It is evident that GGMOs can alter auxin induced elongation and glycosidase activities in different compartments of the cell, however, the mode and site of their action remains unclear. |
| Databáze: | OpenAIRE |
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