Simultaneous extraction of tissue norepinephrine and serotonin
| Autor: | Jerry L. Leeling, Barrie M. Phillips, Paul J. Kraus, Toni L. Hammes |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Brain Chemistry
Guanethidine Carbon Isotopes Serotonin Brain chemistry Chromatography Reserpine Tissue Extracts Butanol Myocardium Uterus Pharmaceutical Science Tritium Rats chemistry.chemical_compound Norepinephrine chemistry Absolute amount Tissue extracts Adrenal Glands Methods Animals Female Spleen Homogenization (biology) |
| Zdroj: | Journal of pharmaceutical sciences. 58(5) |
| ISSN: | 0022-3549 |
| Popis: | A new procedure for the extraction of tissue monoamines combines the homogenization and butanol extraction steps. In addition to the significant reduction in the time necessary for extraction which is characteristic of single-extraction procedures, greater extraction efficiencies are obtained. Hence, the absolute amount of amine available for spectrophotofluorometric determination is greater and the potential error arising from correction for losses during extraction is reduced. The procedure has been routinely employed in this laboratory for more than 2 years, and has proven to be reliable. |
| Databáze: | OpenAIRE |
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