Microwave cell death: Molecular analysis using DNA electrophoresis, PCR amplification and TUNEL
| Autor: | Katsuyoshi Tabuse, Ichiro Mori, Hirotoshi Utsunomiya, Kennichi Kakudo, Emiko Taniguchi, Takashi Ozaki |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Electrophoresis
Male Antigenicity Programmed cell death Gel electrophoresis of nucleic acids Biology Polymerase Chain Reaction Pathology and Forensic Medicine law.invention Rats Sprague-Dawley chemistry.chemical_compound law In Situ Nick-End Labeling Animals Humans Microwaves Polymerase chain reaction TUNEL assay Cell Death nutritional and metabolic diseases DNA General Medicine Middle Aged Molecular biology eye diseases Rats chemistry Biochemistry Apoptosis DNA fragmentation Tumor Suppressor Protein p53 |
| Zdroj: | Pathology International. 59:294-299 |
| ISSN: | 1440-1827 1320-5463 |
| DOI: | 10.1111/j.1440-1827.2009.02368.x |
| Popis: | Microwave cell death (MCD) is unique in that microscopic morphology and antigenicity are well preserved, while enzymatic activity is lost. The aim of the present study was to carry out DNA electrophoresis, polymerase chain reaction (PCR) amplification and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) examination of MCD. DNA electrophoresis indicated only a thick band of large DNA immediately after microwave treatment, which became a smear 6 h later and indicated a delayed type of DNA degradation over 2 weeks. Neither a DNA smear characteristic of oncosis nor DNA ladder patterns characteristic of apoptosis were found in MCD. PCR of p53 in MCD showed a single band at the same level of control of non-microwave treated cells. MCD was negatively stained on TUNEL. These results provide further evidence that MCD is distinct from oncosis and apoptosis, in addition to showing unique morphological and antigenic preservation and enzymatic inactivation. |
| Databáze: | OpenAIRE |
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